芍药甘草复合精油对过氧化氢诱导鼠嗜铬细胞瘤细胞氧化损伤的保护作用研究  

Study on Protective Effect of Shaoyao Gancao Oil on Oxidative Damage of Rat Pheochromocytoma Cells Induced by Hydrogen Peroxide

作　　者：杨心怡 谭晓君 张雪杨 刘薇 刘昆 袁康瑞 朱晖 袁钰 刘小柳 吴都督 赵文昌 陈稚 YANG Xinyi;TAN Xiaojun;ZHANG Xueyang;LIU Wei;LIU Kun;YUAN Kangrui;ZHU Hui;YUAN Yu;LIU Xiaoliu;WU Dudu;ZHAO Wenchang;CHEN Zhi

机构地区：[1]广东医科大学药学院,广东东莞523808 [2]东莞波顿香料有限公司,广东东莞523402 [3]深圳罗湖人民医院医学实验室,广东深圳518001

出　　处：《新中医》2023年第1期1-5,共5页New Chinese Medicine

基　　金：广东省基础与应用基础研究基金项目(2021B1515140020);广东省自然科学基金项目(2018A030307003);广东省中医药局科研项目(20212100);东莞市科技特派员项目(20201800500062);广东医科大学科研基金项目(GDMUZ2020006)。

摘　　要：目的:探讨芍药甘草复合精油(SGO)对过氧化氢(H_(2)O_(2))诱导鼠嗜铬细胞瘤(PC12)细胞氧化损伤的保护机制。方法:采用200μmol/L的H_(2)O_(2)处理PC12细胞建立氧化应激损伤模型。将PC12细胞分为对照组、模型组(200μmol/L H_(2)O_(2))、SGO低浓度组(1μmol/L SGO+200μmol/L H_(2)O_(2))、SGO高浓度组(10μmol/L SGO+200μmol/L H_(2)O_(2))。细胞处理结束后,采用CCK-8法检测细胞存活率;试剂盒检测细胞超氧化物歧化酶(SOD)活力及丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)含量;流式细胞术检测细胞凋亡率;免疫印迹法检测细胞中剪切化半胱氨酸天冬氨酸蛋白激酶-3 (C-Caspase3)、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达。结果:与对照组比较,模型组的细胞存活率、GSH-PX含量、SOD活力及Bcl-2蛋白表达水平明显降低(P<0.05),细胞凋亡率、MDA含量及C-Caspase3、Bax蛋白表达水平明显升高(P<0.05);与模型组比较,SGO低高浓度组的细胞凋亡率、MDA含量及C-Caspase3、Bax蛋白表达水平明显降低(P<0.05),细胞存活率、GSH-PX含量、SOD活力及Bcl-2蛋白表达水平明显升高(P<0.05)。结论:SGO对H_(2)O_(2)诱导的PC12细胞氧化损伤具有保护作用,其作用机制与抗氧化应激损伤、抗细胞凋亡有关。Objective: To discuss the mechanism of Shaoyao Gancao Oil(SGO) protecting pheochromocytoma(PC12) cells of rats from oxidative damage induced by hydrogen peroxide(H_(2)O_(2)).Methods:The PC12 cells were treated with 200μmol/L H_(2)O_(2) and established as the models with oxidative damage. The PC12 cells were divided into the control group,the model group(200 μmol/L H_(2)O_(2)),the lowconcentration SGO group(1 μ mol/L SGO+200 μ mol/L H_(2)O_(2)), and the high-concentration SGO group(10 μmol/L SGO+200 μmol/L H_(2)O_(2)). After the treatment of the PC12 cells,the cell viability was detected by the method of CCK-8;the activity of superoxide dismutase(SOD), as well as the contents of malonic dialdehyde(MDA) and glutathione peroxidase(GSH-PX) of cells were detected by kits;the apoptosis ratio was detected by flow cytometry;the protein expressions of cleaved-caspase3(C-Caspase3), B-cell lymphoma-2(Bcl-2) and Bcl-2 associated X protein(Bax) were detected by Western blot. Results:Compared with those in the control group,the cell viability,GSH-PX content,SOD activity and Bcl-2protein expression level in the model group were significantly decreased(P<0.05),and the apoptosis ratio,MDA content as well as the protein expression levels of C-Caspase3 and Bax were significantly increased(P<0.05);in the low-concentration SGO group and the high-concentration SGO group, the apoptosis ratios,MDA contents as well as the protein expression levels of C-Caspase3 and Bax were significantly decreased(P<0.05),and the protein expression levels of cell viability,GSH-PX contents,SOD activity and Bcl-2 were significantly increased when compared with those in the model group respectively(P<0.05). Conclusion:SGO has a protective effect on H_(2)O_(2)-inducible oxidative damage of PC12 cells,whose mechanism is related to antioxidative stress damage and apoptosis.

关 键 词：氧化损伤 过氧化氢 鼠嗜铬细胞瘤 芍药甘草复合精油 细胞凋亡率 

分 类 号：R285[医药卫生—中药学]