肾纤维大鼠circRNA调控网络构建及靶基因预测  

Construction of circRNA regulatory network and prediction of target genes in renal fiber rats

作　　者：朱芸[1] 杨芳[1] 胡瑚[1] 谭琳燕[1] 梁作辉 ZHU Yun;YANG Fang;HU Hu;TAN Lin-yan;LIANG Zuo-hui(Department of Dermatology,The Sixth Affiliated Hospital of Kunming Medical University,Yuxi 653100,Yunnan Province,China)

机构地区：[1]昆明医科大学第六附属医院皮肤科,云南玉溪653100

出　　处：《中国临床药理学杂志》2023年第3期373-376,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81760136);云南省科技厅-昆明医科大学联合专项重点基金资助项目(2018FE001(-175));云南省高层次卫生计生技术人才培养专项经费资助项目(H-2017028)。

摘　　要：目的 构建肾纤维化大鼠体内circRNA-miRNA-mRNA调控网络,预测肾纤维化潜在的治疗靶点。方法 构建肾纤维大鼠模型,通过全转录组测序及生物信息学分析,筛选显著差异表达的circRNA,并构建其ceRNA调控网络。结果 筛选出17种同时存在于肾纤维化大鼠及老年大鼠中的circRNA,功能富集分析表明这些circRNA的来源基因主要参与FoxO信号通路、黏着斑、内吞作用等。此外,通过构建ceRNA网络发现,这些circRNA还可能通过调控泛素化相关基因参与肾纤维化的发生发展。结论 成功构建circRNA-miRNA-mRNA调控网络,并筛选出10种参与泛素化修饰的基因,这些基因均可能成为肾纤维化的潜在治疗靶点。Objective To construct circRNA-miRNA-mRNA regulatory network in renal fibrosis rats and predict the potential therapeutic targets of renal fibrosis. Methods Renal fiber rat model was established. The significantly abnormal expressed circRNA was screened by whole transcriptome sequencing and bioinformatics analysis, and its ceRNA regulatory network was constructed. Results 17 circRNAs that existed in both renal fibrosis rats and elderly rats were screened out. Functional enrichment analysis showed that these circRNA genes were mainly involved in FoxO signaling pathway, adhesion plaques, and endocytosis. In addition, through the construction of the ceRNA network, it was found that these circRNAs may also be involved in the occurrence and development of renal fibrosis through the regulation of ubiquitin-related genes. Conclusion The circRNA-miRNA-mRNA regulatory network was successfully constructed, and 10 genes involved in ubiquitination modification were screened out, which may become potential therapeutic targets of renal fibrosis.

关 键 词：肾纤维化 环状RNA ceRNA 泛素化 

分 类 号：R97[医药卫生—药品]