刺糖低聚糖润肠通便及调节肠道菌群的功效研究  

Study on the efficacy of rosinose oligosaccharide in nourishing bowel and regulating intestinal microflora

作　　者：宋建忠 李杰 杨婧[3] 陈盈盈 陈章浩 常军民[1] SONG Jian-zhong;LI Jie;YANG Jing;CHEN Ying-ying;CHEN Zhang-hao;CHANG Jun-min(College of Pharmacy,Xinjiang Medical University,Urumqi 830000,Xinjiang Uygur Autonomous Regions,China;Department of Pharmacy,Tumor Hospital Affiliated to Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Regions,China;Department of Pharmacy,People’s Liberation Army Unit Hospital 63650,Urumqi 830000,Xinjiang Uygur Autonomous Regions,China)

机构地区：[1]新疆医科大学药学院,新疆维吾尔自治区乌鲁木齐830000 [2]新疆医科大学附属肿瘤医院药学部,新疆维吾尔自治区乌鲁木齐830011 [3]中国人民解放军63650部队医院药学部,新疆维吾尔自治区乌鲁木齐830000

出　　处：《中国临床药理学杂志》2023年第3期400-404,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82060756);新疆天然药物活性组分与释药技术重点实验室课题资助项目(XJDX1713)。

摘　　要：目的 探究刺糖低聚糖润肠通便及调节肠道菌群的功效。方法 取140只雄性昆明小鼠随机分为空白组、模型组、中药组(600 mg·kg^(-1)麻仁软胶囊)、西药组(2.28 mg·kg^(-1)枸橼酸莫沙必利)和低、中、高剂量实验组(650,1 000和2 000 mg·kg^(-1)刺糖低聚糖)。中药组、西药组和低、中、高剂量实验组分别灌胃给予相应的受试药物,每天1次,连续7 d后,与模型组一同给予5 mg·kg^(-1)盐酸洛哌丁胺,建立小鼠便秘模型。空白组灌胃给予等体积0.9%NaCl。完成造模30 min后,各组小鼠均灌胃给予相应墨汁混悬液。比较各组小肠推进率和排便总重量。另取Balb/c小鼠随机分为空白组和低、中、高剂量实验组,每组10只。按小鼠体质量,低、中、高剂量实验组分别灌胃给予650,1 000和2 000 mg·kg^(-1)刺糖低聚糖,空白组灌胃给予等体积0.9%NaCl,每天1次,连续14 d。以革兰氏染色镜检鉴定计数菌落,对比灌胃前后小鼠粪便菌群计数。结果 中、高剂量实验组和西药组、中药组、模型组、空白组的小肠墨汁推进率分别为(44.55±13.13)%,(57.11±11.60)%,(38.90±6.36)%,(37.31±12.99)%,(38.01±16.30)%和(67.89±9.02)%,粪便总重量分别为(0.12±0.07),(0.28±0.12),(0.31±0.13),(0.22±0.08),(0.20±0.05)和(0.36±0.20)g。高剂量实验组的上述指标与模型组比较,差异均有统计学意义(P<0.01,P<0.05)。高剂量实验组与空白组的双歧杆菌计数分别为(7.58±0.22)和(7.17±0.30)lg(CFU·g^(-1)),差异有统计学意义(P<0.01)。结论 刺糖低聚糖具有良好的润肠通便功效,可增加肠道益生菌数量,调节肠道菌群。Objective To explore the efficacy of rosinose oligosaccharide in nourishing bowel and regulating intestinal flora. Methods A total of 140 male Kunming mice were randomly divided into blank group, model group, traditional Chinese medicine group(600 mg·kg^(-1)seed soft capsule), western medicine group(2.28 mg·kg^(-1)mosapride citrate), experimental-L,-M,-H groups(650, 1 000 and 2 000 mg·kg^(-1)rosinose oligosaccharide). Traditional Chinese medicine group, Western medicine group and experimental-L,-M,-H groups were given corresponding drugs by intragastric administration, once a day and for 7 consecutive days, 5 mg·kg^(-1)loperamide hydrochloride was given together with the model group to establish constipation model in mice. The blank group was given 0.9%NaCl by gavage. 30 min after modeling, each group were given corresponding ink suspension by intragastric administration.Small intestine propulsion rate and total defecation weight were compared in each group. Balb/c mice were randomlydivided into blank group and experimental-L,-M,-H groups, with 10 mice in each group. Experimental-L,-M,-H groups were given 650, 1 000 and 2 000 mg·kg^(-1)rosinose oligosaccharide. Blank group was given 0. 9%NaCl once a day and for 14 days. The bacterial colonies were identified and counted by Gram staining microscopy, andthe fecal bacterial counts of mice were compared before and after intragastric administration.Results The smallintestine ink advance rates of experimental-M,-H groups, western medicine group, traditional Chinese medicinegroup, model group and blank group were( 44. 55 ± 13. 13)%,( 57. 11 ± 11. 60)%,( 38. 90 ± 6. 36)%,(37. 31 ± 12. 99)%,(38. 01 ± 16. 30)% and(67. 89 ± 9. 02)%, the total weights of feces were(0. 12 ± 0. 07),(0. 28 ± 0. 12),(0. 31 ± 0. 13),(0. 22 ± 0. 08),(0. 20 ± 0. 05) and(0. 36 ± 0. 20) g, respectively. Comparedwith the model group, the above indexes in the experimental-H group were significantly different(P< 0. 01,P< 0. 05). TheBifidobacteriumcounts of the experimental-H group and b

关 键 词：刺糖低聚糖 润肠通便 小肠推进率 肠道菌群 双歧杆菌 

分 类 号：R28[医药卫生—中药学]