比格犬关节滑液代谢组学研究  

Metabolomics Study of Joint Synovial Fluid in Beagle Dogs

作　　者：刘清平 黄永平 陈芸 万娟 田松[4] 贺争鸣 胡曼丽 丁一[6] 白兰 张鑫 LIU Qingping;HUANG Yongping;CHEN Yun;WAN Juan;TIAN Song;HE Zhengming;HU Manli;DING Yi;BAI Lan;ZHANG Xin(Gannan Institute of Innovation and Translational Medicine,Key Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases,Ministry of Education,the First Affiliated Hospital of Gannan Medical College,Gannan Medical College,Ganzhou 341000,China;School of Life Sciences,Wuhan University,Wuhan 430072,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China;Renmin Hospital of Wuhan University,Wuhan 430060,China;National Institutes for Food and Drug Control,Beijing 100050,China;Veterinary Medicine College,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]赣南创新与转化医学研究院、心脑血管疾病防治教育部重点实验室、赣南医学院附属第一医院,赣南医学院,赣州341000 [2]武汉大学生命科学学院,武汉430072 [3]中国农业科学院上海兽医研究所,上海200241 [4]武汉大学人民医院,武汉430060 [5]中国食品药品检定研究所,北京100050 [6]华中农业大学动物医学院,武汉430070

出　　处：《实验动物科学》2022年第6期5-14,共10页Laboratory Animal Science

基　　金：国家自然科学基金(82000538);河南慈善基金(GDXZ2022008,GDXZ2022006)。

摘　　要：目的基于代谢图谱作为量化关节腔滑液内代谢物的工具,发现比格犬关节发育过程中的代谢规律。方法收集幼年和成年比格犬关节滑液,运用液质联用(LC-MS)非靶向代谢组学手段,分析不同年龄段比格犬关节腔滑液代谢组学特征及其与发育的相关性。结果与同性别成年比格犬相比,幼年雄性比格犬关节滑液中参与蛋白质、氨基酸、矿物质代谢和ABC转运的代谢物含量较高;幼年雌性比格犬中与氨基酸、碳水化合物代谢和ABC转运相关的代谢物含量较高。结论氨基酸、蛋白质、矿物质及碳水化合物的代谢能力与犬骨关节发育及关节健康密切相关。Objective To analyze and compare metabolomics characteristics of joint synovial fluid between juvenile and adult beagle dogs.Method The joint synovial fluids were collected from 18 juvenile and 16 adult beagle dogs.Samples were subjected to liquid chromatography and mass spectrometry(LC-MS)-based nontargeted metabolomics analyses.Result Compared with that of adult dogs,male juvenile animals showed higher levels of metabolites related to amino acid biosynthesis,protein digestion and absorption,mineral absorption,and ABC transporter.In female animals,the contents of metabolites related to amino acid,carbohydrate metabolism and ABC transport were higher in juvenile than adult dogs.Conclusion The bone joint development and health are closely related to metabolic events of amino acids,proteins,minerals and carbohydrates.

关 键 词：比格犬 关节滑液 代谢组学 关节发育 关节养护 

分 类 号：Q95-3[生物学—动物学]