应用24个微卫星标记分析SD和Wistar大鼠的遗传多样性  

Genetic Diversity of SD and Wistar Rats was Analyzed by Using 24 Microsatellite Markers

作　　者：李欢 魏杰[1] 左琴[1] 王洪[1] 周佳琪 光姣娜 范涛[1] 付瑞[1] 刘佐民[1] 岳秉飞[1] LI Huan;WEI Jie;ZUO Qin;WANG Hong;ZHOU Jiaqi;GUANG Jiaona;FAN Tao;FU Rui;LIU Zuomin;YUE Bingfei(National Institutes for Food and Drug Control,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院,北京102629

出　　处：《实验动物科学》2022年第6期32-38,共7页Laboratory Animal Science

基　　金：基于SNP技术建立我国常用实验动物遗传质量评价技术体系(2021YFF0703200)。

摘　　要：目的 通过SD和Wistar大鼠群体遗传多样性分析,为其质量控制提供基础数据。方法 采用微卫星DNA标记技术,选取分布于21条染色体上的24个微卫星位点分别对12对SD大鼠和Wistar大鼠的DNA样本进行PCR扩增,所得扩增产物经二代测序分型,再利用PopGen32软件、Littleprogram0.6和GenePop分别进行等位基因、平均杂合度、多态信息含量及遗传平衡检验参数的计算。结果 SD和Wistar大鼠群体分别在24个微卫星位点检测到平均等位基因数3、2.95个,平均杂合度为0.434 4、0.426 1,平均多态信息含量分别为0.38、0.37,分别有10、9个位点偏离了Hardy-Weinberg遗传平衡,其中7、4个位点表现为杂合子缺失。结论 SD和Wistar大鼠封闭群遗传多样性略低于理想封闭群水平,需要在种群繁育中注重科学管理监测,避免近交。Objective To provide basic data for quality control by analyzing the genetic diversity of SD and Wistar rat populations.Method Using microsatellite DNA labeling technology,24 microsatellite loci distributed on 21 chromosomes were selected for PCR amplification of DNA samples from 12 pairs of SD rats and Wistar rats,respectively.The amplified products were classified by second-generation sequencing.Then PopGen32 software,Littleprogram0.6 and GenePop software were used to calculate alleles,average heterozygosity,polymorphism information content and genetic balance test parameters.Result The average number of alleles of 24 microsatellite loci in SD rats and Wistar rats was 3 and 2.95,respectively.The average heterozygosity was 0.434 4 and 0.426 1,and the average polymorphism information content was 0.38 and 0.37,respectively.There were 10 and 9 loci deviating from the Hardy-Weinberg equilibrium.Among them,7 and 4 loci showed heterozygous deletion.Conclusion The genetic diversity of SD and Wistar rat closed populations is slightly lower than that of ideal closed populations.It is necessary to pay attention to scientific management and monitoring in population breeding to avoid inbreeding.

关 键 词：微卫星 封闭群 WISTAR大鼠 SD大鼠 遗传多样性 

分 类 号：Q95-3[生物学—动物学]