银杏叶色素类化合物的提取分离及结构鉴定  

Extraction,Isolation and Structure Identification of Pigment Compounds from Ginkgo biloba Leaves

作　　者：沈红 张昌伟[1,3] 陈虹霞 陶冉[1,3] 蒋建新[2] 王成章[1,3] SHEN Hong;ZHANG Changwei;CHEN Hongxia;TAO Ran;JIANG Jianxin;WANG Chengzhang(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;College of Materials Science and Technology,Beijing Forestry University,Beijing 100083,China;Research Institute of Forestry New Technology,CAF,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042 [2]北京林业大学材料科学与技术学院,北京100083 [3]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2023年第1期79-88,共10页Chemistry and Industry of Forest Products

基　　金：江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-202008);国家自然科学基金资助项目(32101473);中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2020SY039)。

摘　　要：以银杏鲜叶(样品1)、银杏干叶(样品2)和银杏陈叶(样品3)为原料,制备了3个样品的乙醇提取物,采用HPLC-DAD-MS法分析3个样品中银杏叶色素类化合物的组成;通过液液萃取、硅胶柱层析和HPLC制备色谱等技术手段对样品2和样品3的银杏叶色素类化合物进行了分离纯化和结构鉴定。从3个样品中初步推测出24种银杏叶色素类化合物,其中叶绿素类化合物17种,类胡萝卜素类化合物7种。从样品2和样品3的乙醇提取物浸膏中分离纯化出10种银杏叶色素单体化合物,通过质谱、紫外光谱、红外光谱和核磁共振氢谱数据分析,并结合文献对照鉴定化合物分别为:β-胡萝卜素(A)、脱镁叶绿素a(B)、脱镁叶绿素b(C)、叶黄素(D)、植基紫红素18a(E)、焦脱镁叶绿素a(F)、13^(2)-羟基脱镁叶绿素a(G)、13^(2)-羟基脱镁叶绿素a′(H)、13^(2)-羟基脱镁叶绿素b(I)、13^(2)-羟基脱镁叶绿素b′(J),其中E、F、G、H、I、J为首次从银杏叶中分离得到。Ethanol extracts of three samples were prepared from Ginkgo biloba L.fresh leaves(sample 1),dry leaves(sample 2)and old leaves(sample 3).HPLC-DAD-MS method was used to analyze the pigment compounds in three G.biloba leaves samples.The separation,purification and structure identification of ginkgo leaf pigment compounds in sample 2 and sample 3 were conducted by liquid-liquid extraction,silica gel column chromatography,HPLC preparative chromatography,etc.From the three samples,24 kinds of pigment compounds in G.biloba leaves were preliminarily speculated,including 17 kinds of chlorophyll compounds and 7 kinds of carotenoid compounds.Ten monomer pigment compounds in G.biloba leaves were isolated and purified from ethanol extract of sample 2 and sample 3.They were identified asβ-carotene(A),pheophytin a(B),pheophytin b(C),lutein(D),phy-purpurin 18a(E),pyropheophytin a(F),13^(2)-OH-pheophytin a(G),13^(2)-OH-pheophytin a′(H),13^(2)-OH-pheophytin b(I),and 13^(2)-OH-pheophytin b′(J),respectively,by mass spectrometry,ultraviolet spectroscopy,infrared spectroscopy and proton nuclear magnetic resonance spectroscopy combined with literature comparison.Among them,E,F,G,H,I and J were isolated from G.biloba leaves for the first time.

关 键 词：银杏叶 色素 提取 分离 结构鉴定 

分 类 号：TQ35[化学工程]