荧光光谱法研究单壁碳纳米管与牛血红蛋白的相互作用  

Fluorescence Spectroscopic Investigation of Interaction of Single-Walled Carbon Nanotubes with Bovine Hemoglobin

作　　者：杨兴 谭惠心 章弘扬 胡坪 张敏 YANG Xing;TAN Huixin;ZHANG Hongyang;HU Ping;ZHANG Min(Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism,Engineering Research Center of Ministry of Education for Pharmaceutical Engineering and Process Chemistry,School of Pharmacy,Shanghai 200237,China;Shanghai Key Laboratory of Functional Materials Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市细胞代谢光遗传学技术前沿科学研究基地,上海200237 [2]华东理工大学化学与分子工程学院,上海市功能性材料化学重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2023年第1期12-18,共7页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(81573397)。

摘　　要：选取了单壁碳纳米管(SWCNTs)及两种分离后的单一手性单壁碳纳米管(6,5)-SWCNTs、(8,3)-SWCNTs分别与牛血红蛋白(BHb)结合,通过荧光光谱法分析SWCNTs与BHb的相互作用。结果表明,SWCNTs对BHb的荧光猝灭为动态猝灭与静态猝灭结合的机制,(6,5)-SWCNTs和(8,3)-SWCNTs对BHb的荧光猝灭属于静态猝灭的机制;298 K下BHb与3种SWCNTs的结合常数大小顺序如下:SWCNTs>(6,5)-SWCNTs>(8,3)-SWCNTs;范德华力、氢键和疏水作用是相互作用中的主要作用力。Single-walled carbon nanotubes(SWCNTs) can combine with proteins in organisms, creating potential biosafety risks. In this paper, SWCNTs and two separated single-chiral single-walled carbon nanotubes((6,5)-SWCNTs,(8,3)-SWCNTs) were combined with bovine hemoglobin(BHb), respectively, and interaction between SWCNTs and BHb was analyzed by fluorescence spectroscopy. The results showed that the fluorescence quenching of BHb by SWCNTs was stemmed from the combination of dynamic quenching and static quenching. The fluorescence quenching of BHb by single chiral(6,5)-SWCNTs and(8,3)-SWCNTs was static quenching. At 298 K, the order of the binding constants of BHb with different SWCNTs were as follows: SWCNTs >(6,5)-SWCNTs >(8,3)-SWCNTs. Van der Waals force, hydrogen bond and hydrophobic interaction were the main forces in the interaction.

关 键 词：单壁碳纳米管 牛血红蛋白 相互作用 荧光光谱 猝灭 

分 类 号：O657.3[理学—分析化学]