免疫细胞介导炎症反应对糖尿病大鼠动脉内皮细胞凋亡的影响机制研究  

Study on mechanism of immune cell mediated inflammation response on apoptosis of arterial endothelial cells in diabetic rats

作　　者：谢雯雯 林玉平 何志凌 范冠杰 XIE Wenwen;LIN Yuping;HE Zhiling;FAN Guanjie(Department of Endocrinology,Second Clinical Medical College,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510000,China)

机构地区：[1]广州中医药大学第二临床医学院内分泌科,广州510000 [2]广州中医药大学第二临床医学院心血管科,广州510000

出　　处：《中国免疫学杂志》2023年第1期34-38,共5页Chinese Journal of Immunology

基　　金：广东省中医院中医药蛋白质组研究中心项目(YN2018DB03)。

摘　　要：目的:探究T辅助细胞17(Th17)/调节性T细胞(Treg)介导炎症反应对糖尿病(DM)大鼠动脉内皮细胞凋亡影响机制。方法:腹腔注射链脲佐菌素建立大鼠DM模型,将DM大鼠分为DM组和治疗组(n=12),另取12只健康大鼠作为对照组。比较各组大鼠的血糖、主动脉损伤情况、血清IL-6、TNF-α和动脉内皮细胞中凋亡标志蛋白Caspase3和Bcl-2水平。并通过流式细胞术检测外周血中Th17/Treg水平。结果:建模后大鼠血糖均高于16.7 mmol/L,干预后治疗组的空腹血糖水平显著低于DM组(P<0.05)。DM组的主动脉血管壁厚度(54.47±2.61)μm、血清IL-6(19.24±2.21)pg/mg和TNF-α(39.46±3.56)pg/mg水平、动脉内皮细胞中Caspase3 mRNA和Bcl-2 mRNA和蛋白表达量、Th17水平(2.72±0.38)%和Th17/Treg比例(1.23±0.19)显著高于对照组,Treg水平(2.21±0.38)%显著低于对照组(P<0.05)。治疗组动脉壁厚度(46.82±2.72)μm、IL-6(13.86±1.87 pg/mg、TNF-α(22.35±3.04)pg/mg、Caspase3 mRNA和Bcl-2 mRNA和蛋白表达量、Th17水平(1.50±0.20)%和Th17/Treg比例(0.46±0.07)显著低于DM组,Treg(3.26±0.52)%水平显著高于DM组(P<0.05)。结论:Th17/Treg免疫细胞介导炎症反应可能参与了DM引起的动脉内皮细胞凋亡。Objective:To investigate the mechanism of T help 17 cells(Th17)/regulatory T cells(Treg)mediated inflammatory response on the apoptosis of arterial endothelial cells in diabetes mellitus(DM)rats.Methods:The rat model of DM was established by intraperitoneal injection of streptozotocin.DM rats were divided into DM group and treatment group(n=12).Another 12healthy rats were taken as control group.The blood glucose,aortic injury,serum IL-6,TNF-αand the levels of apoptosis marker proteins Caspase3 and Bcl-2 in arterial endothelial cells were detected in each group of rats.The Th17/Treg levels in peripheral blood were detected by flow cytometry.Results:After modeling,the blood glucose of rats was above 16.7 mmol/L.After the intervention,the fasting blood glucose level of the treatment group was significantly lower than that of DM group(P<0.05).Aortic vessel wall thickness(54.47±2.61)μm,serum IL-6(19.24±2.21)pg/mg and TNF-α(39.46±3.56)pg/mg levels,Caspase3 mRNA and Bcl-2 mRNA and protein in arterial endothelial cells,Th17 level(2.72±0.38)%and Th17/Treg ratio(1.23±0.19)in DM group were significantly higher than those in control group,and the Treg level(2.21±0.38)%was significantly lower than that in control group(P<0.05).The arterial wall thickness of treatment group was(46.82±2.72)μm,IL-6(13.86±1.87)pg/mg,TNF-α(22.35±3.04)pg/mg,Caspase3mRNA and Bcl-2 mRNA and protein expression,Th17 level(1.50±0.20)%and Th17/Treg ratio(0.46±0.07)were significantly lower than those in DM group,and the Treg(3.26±0.52)%level was significantly higher than that in DM group(P<0.05).Conclusion:Th17/Treg immune cell-mediated inflammation may be involved in the apoptosis of arterial endothelial cells caused by DM.

关 键 词：糖尿病 动脉内皮细胞 T辅助细胞 调节性T细胞 炎症反应 凋亡 

分 类 号：R587.1[医药卫生—内分泌]