50份热带睡莲材料的耐寒性评价  

Evaluation on cold tolerance of 50 tropical water lily materials

作　　者：迟明宏 程哲 杨志娟 王彦杰[1,2,3] 金奇江 杨梅花 王雪莲[5] 徐迎春[1,2,3] CHI Minghong;CHENG Zhe;YANG Zhijuan;WANG Yanjie;JIN Qijiang;YANG Meihua;WANG Xuelian;XU Yingchun(Nanjing Agricultural University,Nanjing 210095,China;College of Horticulture,b.Sanya Research Institute,Nanjing 210095,China;Key Laboratory of Flower Biology and Germplasm Creation,Ministry of Agriculture and Rural Affairs,Nanjing 210095,China;Sanya Nanfan Research Institute,Hainan University,Sanya 572025,China;Agriculture College,Shihezi University,Shihezi 832099,China)

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]南京农业大学三亚研究院,江苏南京210095 [3]南京农业大学农业农村部花卉生物学与种质创制重点实验室,江苏南京210095 [4]海南大学三亚南繁研究院,海南三亚572025 [5]石河子大学农学院,新疆石河子832099

出　　处：《植物资源与环境学报》2023年第1期39-49,共11页Journal of Plant Resources and Environment

基　　金：国家自然科学基金新疆联合基金项目(U1803104;U2003113);海南省自然科学基金高层次人才项目(321RC670);中央高校基本科研业务费专项资金项目(KYZZ2022004)。

摘　　要：本研究对50份热带睡莲材料进行4℃、0℃、-6℃和-12℃的人工低温处理,测定了叶片相对电导率和半致死温度(LT 50),并对可溶性糖、丙二醛(MDA)和游离脯氨酸含量以及超氧化物歧化酶(SOD)活性进行了比较,并利用相关性分析和k均值聚类分析等方法对50份热带睡莲材料的耐寒性进行分级。结果表明:LT 50值可用于初步判断不同热带睡莲材料的耐寒性,其中,大部分热带睡莲材料的LT 50值介于-5℃~0℃范围内,‘江南风韵’(‘Southern Charm’)的LT 50值最低(-5.85℃),‘潘燮’(‘Poonsub’)的LT 50值最高(3.69℃)。随着温度降低,可溶性糖、MDA和游离脯氨酸含量以及SOD活性的变化趋势整体可分为3类:第1类各低温处理间的上述4个指标差异均不显著,总体变幅不大;第2类和第3类各低温处理间的上述4个指标的变化趋势各异且存在显著差异,总体变幅较大。相关性分析结果显示:热带睡莲材料的LT 50值与可溶性糖含量、MDA含量和游离脯氨酸含量呈极显著正相关,与SOD活性呈极显著负相关。进一步选取LT 50值、MDA含量、可溶性糖含量和SOD活性作为建立热带睡莲耐寒性评价体系的指标。k均值聚类分析结果显示:50份热带睡莲材料可分为4类,即不耐寒型、弱耐寒型、中等耐寒型和强耐寒型。综上所述,热带睡莲会通过多种生理生化反应来应对低温胁迫,总体上看,耐寒性越强的材料的可溶性糖和MDA含量越低,SOD活性越高;埃及白睡莲〔Nymphaea lotus(Linn.)Willdenow〕表现出强耐寒性,可以考虑作为耐寒性育种的优良亲本。In this study,50 tropical water lily materials were subjected to artificial low temperature treatments at 4℃,0℃,-6℃,and-12℃,relative conductivity and semi-lethal temperature(LT 50)of the leaves were determined,contents of soluble sugar,malondialdehyde(MDA),and free proline and superoxide dismutase(SOD)activity were compared,and the cold tolerance of 50 tropical water lily materials was graded by using correlation analysis and k-means cluster analysis,etc.The results show that LT 50 values can be used to preliminarily judge the cold tolerance of different tropical water lily materials,in which,the LT 50 values of most tropical water lily materials are within the range of-5℃-0℃,the LT 50 value of‘Southern Charm’is the lowest(-5.85℃),while that of‘Poonsub’is the highest(3.69℃).With the decrease of temperature,the variation tendency of contents of soluble sugar,MDA,and free proline and SOD activity can be divided into three categories:the differences in above-mentioned 4 indexes among each low temperature treatment of the first category are all not significant,and the overall variation degree is not great;the variation tendency of above-mentioned 4 indexes among each low temperature treatment of the second and third categories are different and there are significant differences,and the overall variation degree is relatively great.The correlation analysis result shows that LT 50 values of tropical water lily materials show extremely significant positive correlations with contents of soluble sugar,MDA,and free proline,but show extremely significant negative correlation with SOD activity.LT 50 value,MDA content,soluble sugar content,and SOD activity are further selected as indexes to establish a cold tolerance evaluation system for tropical water lilies.The k-means cluster analysis result shows that 50 tropical water lily materials can be divided into four categories,namely non-cold tolerant type,weak cold tolerant type,medium cold tolerant type,and strong cold tolerant type.Taken together,tropic

关 键 词：热带睡莲 耐寒性 半致死温度(LT 50) 生理生化指标 相关性分析 聚类分析 

分 类 号：Q945.78[生物学—植物学] S682.32[农业科学—观赏园艺]