槲皮素对过氧化氢诱导的人脐静脉内皮细胞AKT/mTOR信号通路及自噬的影响  

Effect of quercetin on hydrogen peroxide-induced AKT/mTOR signaling pathway and autophagy in human umbilical vein endothelial cells

作　　者：宋涛[1] 官泽宇[1] 徐超[1] 朱二畅 蔡瑶 卢冉[1] 余朝文[1] SONG Tao;GUAN Ze-yu;XU Chao;ZHU Er-chang;CAI Yao;LU Ran;YU Chao-wen(Department of Vascular Surgery,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)

机构地区：[1]蚌埠医学院第一附属医院血管外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2023年第2期141-144,共4页Journal of Bengbu Medical College

基　　金：蚌埠医学院自然科学研究重点项目(BYKY2019071ZD)。

摘　　要：目的:分析槲皮素对人脐静脉内皮细胞自噬和增殖的影响,探讨其与AKT/mTOR信号通路调控的关系及机制。方法:培养人脐静脉内皮细胞,MTT法检测细胞活力,MDC染色法检测自噬泡水平,Western印迹检测自噬相关蛋白水平,运用3-MA研究槲皮素对自噬和细胞增殖的影响。结果:槲皮素能增加人脐静脉内皮细胞活力(P<0.01),上调细胞中MDC染色标记的荧光颗粒,并伴随浓度的上调明显增加(P<0.01),能促进脐静脉内皮细胞中自噬蛋白LC3Ⅱ/LC3Ⅰ水平表达(P<0.01),降低p-AKT/AKT、p-mTOR/mTOR水平(P<0.01)。与槲皮素组比较,槲皮素+3-MA组细胞活力和LC3Ⅱ/LC3Ⅰ均明显降低(P<0.05),p-mTOR/mTOR明显增加(P<0.05)。结论:槲皮素可通过抑制AKT/mTOR信号通路诱导细胞自噬来调控脐静脉内皮细胞增殖。Objective:To analyze the effect of quercetin on autophagy and proliferation of human umbilical vein endothelial cells and its relationship with AKT/mTOR signaling pathway.Methods:MTT method was used to detect cell viability.MDC staining was used to detect the level of autophagy vesicles.Western blotting was used to detect the levels of autophagy-related proteins,and 3-MA was used to study the effect of quercetin on autophagy and cell proliferation.Results:Quercetin increased the viability of human umbilical vein endothelial cells and up-regulated the fluorescent particles labeled by MDC staining in the cells followed with the increase in concentration,which promoted the expressions of autophagy protein LC3Ⅱ/LC3Ⅰin umbilical vein endothelial cells and reduce the levels of p-AKT/AKT and p-mTOR/mTOR(P<0.01).Compared with the quercetin group,the cell viability and LC3Ⅱ/LC3Ⅰof the quercetin+3-MA group were significantly reduced,and p-mTOR/mTOR was significantly increased(P<0.05).Conclusions:Quercetin can regulate the proliferation of umbilical vein endothelial cells by inhibiting the AKT/mTOR signaling pathway and inducing autophagy.

关 键 词：槲皮素 AKT/mTOR信号通路 自噬 脐静脉内皮细胞 细胞增殖 

分 类 号：R285[医药卫生—中药学]