分子检测和血清学指标检测对儿童肺炎支原体肺炎早期诊断的价值分析  

Analysis on Value of Molecular Tests and Serological Index Tests in Early Diagnosis of Mycoplasma Pneumoniae Pneumonia in Children

作　　者：杨红叶[1] Yang Hong-ye(Department of Pediatrics,Jiangyin Third People's Hospital,Wuxi 214400,Jiangsu Province,China)

机构地区：[1]江阴市第三人民医院儿科,江苏无锡214400

出　　处：《中国社区医师》2023年第3期69-71,共3页Chinese Community Doctors

摘　　要：目的:分析分子检测和血清学检测对儿童肺炎支原体肺炎(MPP)的早期诊断价值。方法:选取2021年9月-2022年12月江阴市第三人民医院儿科收治的25例MPP患儿作为MPP组,另选取25例健康儿童作为非MPP组。均进行分子检测和血清学检测,以组织活检作为金标准。比较MPP组和非MPP组各指标的差异性,分析白细胞介素6(IL-6)、白细胞介素10(IL-10)、C反应蛋白(CRP)、血小板压积(PCT)与MPP的相关性,研究IL-6/IL-10、CRP/PCT对MPP的诊断价值,并探讨分子检测和血清学检测对MPP的早期诊断效能。结果:MPP组RNA、DNA纯度低于非MPP组,差异有统计学意义(P<0.05);MPP组IL-6、IL-10、CRP、PCT水平均高于非MPP组,差异有统计学意义(P=0.000);联合检测的确诊率高于血清学检测,差异有统计学意义(P=0.042);联合检测与血清学检测灵敏度、准确率、阳性预测值比较,差异无统计学意义(P>0.05)。结论:血清学检测虽能诊断儿童细菌感染的严重程度,但在鉴别细菌、病毒及非典型微生物感染能力上存在一定缺陷,仍需配合分子学进一步测定,联合以RT-PCR为代表的分子检测,可有效鉴别患儿早期肺炎,为实际临床治疗提供可靠的参考依据。Objective:To analyze the early diagnostic value of molecular tests and serology tests for mycoplasma pneumoniae pneumonia(MPP) in children.Methods:A total of 25 children with MPP admitted to Department of Pediatrics,Jiangyin Third People’s Hospital from September 2021 to December 2022 were selected as MPP group.Another 25 healthy children were selected as the non-MPP group.All children were tested by molecular tests and serology tests,and tissue biopsy was taken as the gold standard.The differences of each indicator between MPP group and non-MPP group were compared.The correlation of interleukin 6(IL-6),interleukin 10(IL-10),C-reactive protein(CRP),plateletcrit(PCT) with MPP was analyzed.The diagnostic value of IL-6/IL-10 and CRP/PCT for MPP was studied.The early diagnostic efficacy of molecular tests and serology tests for MPP was explored.Results:The RNA and DNA purity in the MPP group were lower than those in the non-MPP group,and the difference was statistically significant(P<0.05).The levels of IL-6,IL-10,CRP and PCT in the MPP group were higher than those in the non-MPP group,and the differences were statistically significant(P=0.000).The diagnosis rate of combined tests was higher than that of serology tests,and the difference was statistically significant(P=0.042).There was no significant difference in the sensitivity,accuracy,and positive predictive value between combined tests and serology tests(P>0.05).Conclusion:Serology tests can diagnose the severity of bacterial infection in children,but it has some defects in the identification ability of bacteria,viruses and atypical microbial infection,which still needs to cooperate with molecular tests for further determination.The combination with molecular tests represented by RT-PCR can effectively identify children with early pneumonia,and provide a reliable reference basis for practical clinical treatment.

关 键 词：分子检测 血清学检测 儿童肺炎支原体肺炎 

分 类 号：R725.6[医药卫生—儿科]