基于网络药理学探讨某医院制剂治疗腱鞘炎的作用机制  

Study on the Mechanism of a Hospital Preparation for Tenosynovitis Based on Network Pharmacology

作　　者：区通源 黄会平 张昊亮 马建春 OU Tongyuan;HUANG Huiping;ZHANG Haoliang;MA Jianchun(The first Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou,Guangdong 510080,China)

机构地区：[1]广东药科大学附属第一医院,广东广州510080

出　　处：《今日药学》2022年第12期919-922,927,共5页Pharmacy Today

摘　　要：目的运用网络药理学分子对接方法分析院内制剂白药膏治疗腱鞘炎的作用机制。方法分别确定白药膏和腱鞘炎的靶点,生成药材和疾病的交集基因和Hub基因,最后进行基因本体功能富集分析和京都基因与基因组百科全书通路注释富集分析,确定白药膏治疗腱鞘炎的生物过程和通路,运用分子对接方法验证化合物和基因的结合能力。结果白药膏和腱鞘炎的交集基因为26个,IL-6、TNF、CCL2、IL-10等为Hub基因;GO功能富集中细胞因子受体结合、细胞因子活性等为主要的生物过程;KEGG通路注释富集中TNF信号通路、白细胞介素17信号通路等为主要通路。分子对接显示核心靶点与化合物有较强亲和力。结论白药膏治疗腱鞘炎主要通过作用与IL-6、TNF靶点发挥作用,初步阐明其作用机制,为院内制剂的深入研究提供思路。OBJECTIVE To analyze the mechanism of Baiyao ointment in treating tenosynovitis by network pharmacologyand molecular docking.METHODS The targets of baiyao ointment and tenosynovitis were determined respectively,and the intersection genes and Hub genes were generated.GO function concentrates and KEGG pathway annotation were conducted to determine the biological process and pathway of baiyao ointment in the treatment of tenosynovitis.RESULTS There were 26 intersection genes between Baiyao ointment and tenosynovitis,IL-6,TNF,CCL2,IL-10 were Hub genes.Cytokine receptor binding and cytokine activity were the main biological processes of GO function concentrates.TNF signaling pathway,interleukin 17 signaling pathway and other major pathways were KEGG pathway annotation.Molecular docking showed that the core target had strong affinity with the compound.CONCLUSION Baiyao ointment in the treatment of tenosynovitis mainly plays a role by acting on IL-6 and TNF targets.The mechanism of action is preliminarily elucidated,which provides ideas for the further study of hospital preparations.

关 键 词：网络药理学 白药膏 腱鞘炎 院内制剂 

分 类 号：R966[医药卫生—药理学]