两种2-苯基吡啶铱配合物的合成及其在细胞中的应用  

Synthesis of Two 2-Phenylpyridine Iridium Complexes and Their Applications in Cells

作　　者：王亚轩 于坤 沈舒婷 王慧 WANG Yaxuan;YU Kun;SHEN Shuting;WANG Hui*(School of Pharmacy,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002

出　　处：《合成化学》2023年第2期161-166,共6页Chinese Journal of Synthetic Chemistry

基　　金：国家级大学创新创业训练计划项目(202110368056);皖南医学院大学生科研资助项目(WK2021XS44);安徽省大学生创新创业教育训练计划项目(S202110368196)。

摘　　要：以4-(2-吡啶基)-苯甲醛、邻菲罗啉、对羟基苯甲醛、香草醛、溴丁烷和三氯化铱水合物为原料,合成了两种铱配合物IrL1和IrL2,其结构经核磁共振氢谱、碳谱、质谱和傅里叶红外光谱表征。研究了两种配合物在不同溶剂中的紫外可见吸收光谱和荧光发射光谱。结果表明:配合物IrL1和IrL2在乙醇中的荧光强度高于其它溶剂,最大发射波长位于542 nm。采用MTT实验和共聚焦显微成像技术研究了配合物IrL1和IrL2在HepG2细胞中的应用效果。结果表明:两种配合物细胞毒性较低,能够着色于固定细胞的细胞质中。Two iridium complexes IrL1 and IrL2 were synthesized from 4-(2-pyridyl)-benzaldehyde, o-phenanthroline, p-hydroxybenzaldehyde, vanillin, bromobutane and IrCl3·3H2O, which were characterized by ~1H NMR,13C NMR, MALDI-TOF and IR. UV-Vis absorption and fluorescence emission spectra of these two complexes in different solvents were investigated. The results demonstrated that the fluorescence intensity of IrL1 and IrL2 in ethanol was higher than that in other solvents, and the maximum emission wavelength was about 542 nm. The application of complexes IrL1 and IrL2 in HepG2 cells was studied by MTT assay and confocal microscopy. The results indicated that these two complexes possess low cytotoxicity, which could be stained in the cytoplasm of the fixed cells.

关 键 词：2-苯基吡啶 铱配合物 细胞成像 光物理性质 合成 邻菲罗啉 

分 类 号：O614.8[理学—无机化学]