姜黄素调控p38MAPK通路改善糖尿病心肌病的机制研究  

The Protective Effect of Curcuminon Diabetic Cardiomyopathy by p38MAPK Pathway

作　　者：寿曦缘 朱晓明[1] 张飞雪[2] 郭霜[2] SHOU Xi-yuan;ZHU Xiao-ming;GUO Shuang(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院糖尿病心脑血管病变湖北省重点实验室

出　　处：《湖北科技学院学报（医学版）》2023年第1期1-4,共4页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院糖尿病心脑血管病变湖北省重点实验室糖尿病专项基金(2022TNB08,2022TNB09);湖北科技学院校内培育科研项目(2021-22X29)。

摘　　要：目的探讨姜黄素(Cur)通过调控p38MAPK通路抑制炎症反应改善糖尿病心肌病的作用机制。方法50只雄性SPF级C57BL/6小鼠,随机选取20只作为正常组,剩余30只小鼠给予高糖、高脂饲料喂养4周后,连续5d腹腔注射50mg/(kg·d)链脲佐菌素,以空腹血糖≥11.1mol/L作为糖尿病造模成功标准。正常组小鼠随机分为正常对照组(Con组)和姜黄素对照组(Cur组),糖尿病小鼠随机分为糖尿病组(DM组)和姜黄素给药组(DM+Cur组)。实验结束后,ELISA法检测各组小鼠心肌组织匀浆液中炎症因子TNF-α和IL-6含量;免疫荧光法检测各组小鼠心肌组织中TNF-α水平;Western blot法检测各组小鼠心肌组织中p-p38、p38和MCP-1蛋白表达。结果Cur可明显逆转糖尿病所致小鼠心肌组织匀浆液中TNF-α和IL-6的变化,减少心肌组织中TNF-α的表达,下调糖尿病小鼠心肌组织中p38MAPK磷酸化水平和MCP-1蛋白的表达。结论姜黄素通过抑制炎症反应改善小鼠糖尿病心肌病,其作用机制可能与抑制p38MAPK信号转导通路有关。Objective To investigate the effect of Curcumin(Cur)on myocardial tissue in diabetic mice by improving inflammation.Methods 50 male SPF C57BL/6 mice were randomly divided into normal control group(Con group),curcumin control group(Cur group),diabetes group(DM group)and curcumin administration group(DM+Cur group).The model of diabetes was established by intraperitoneal injection of streptozotocin at dose of 50 mg/(kg·d)for a consecutive 5 days after high sugar and high fat diet for 4 weeks.Fasting blood glucose≥11.1mmol/L was taken as the success criterion of diabetes modeling.The contents of TNF-αand IL-6 in myocardial tissue were detected by ELISA,the expression of TNF-αin myocardium of mice was measured by immunofluorescence staining,and the levels of p38MAPK,p-p38MAPK and MCP-1 protein in myocardium of mice were detected by Western blot.Results Curcumin significantly decreased the levels of TNF-αand IL-6 in diabetic mouse myocardial tissue,inhibited high glucose-induced phosphorylation of p38MAPK and the expression of MCP-1.Conclusion Curcumin has protective effect on myocardial tissue in diabetic mice by improving inflammation and the mechanisms maybe involved in the regulation of p38MAPK signaling pathway.

关 键 词：姜黄素 糖尿病心肌病 炎症反应 P38MAPK 

分 类 号：R966[医药卫生—药理学]