β-榄香铜酸对人食管癌细胞Eca-109的抑制作用  

Inhibitory Effect ofβ-Elemenic Acid on Human Esophageal Cancer Cell Eca-109

作　　者：魏瑶璐 李燕[1] 黄圣 刘佩 陈烨楠 曾凤娇 宁志丰[2] 刘复兴[2] WEI Yao-lu;NING Zhi-feng;LIU Fu-xing(School of Pharmacy,Xianning Medical College,Hubei University of Science and Technology,Xianning Hubei 437100,China)

机构地区：[1]湖北科技学院医学部药学院,湖北咸宁437100 [2]湖北科技学院医学部基础医学院

出　　处：《湖北科技学院学报（医学版）》2023年第1期15-20,共6页Journal of Hubei University of Science and Technology(Medical Sciences)

基　　金：湖北科技学院五官专项(202002);2022年湖北省重点研发计划(大健康领域支持地方专项)。

摘　　要：目的探究β-榄香铜酸对人食管癌细胞Eca-109增殖、迁移、侵袭的影响及作用机制。方法使用不同浓度的β-榄香铜酸(0、20、40、60μmol/L)处理Eca-109细胞,首先进行MTT、平板克隆、细胞划痕、transwell迁移、transwell侵袭实验检测β-榄香铜酸对Eca-109的细胞增殖、迁移、侵袭的影响;之后在60μmol/L浓度组使用铁死亡抑制剂Ferrostatin-1(Fer-1)干预探索其作用机制。使用试剂盒检测Eca-109细胞中Fe^(2+)、ROS、lipid ROS、GSH、MDA含量,使用Western blot实验检测Eca-109细胞中GPX4、PTGS2和4-HNE的蛋白表达。结果β-榄香铜酸可呈剂量依赖性抑制Eca-109的细胞增殖、克隆形成、迁移、侵袭,激活Eca-109中铁死亡通路蛋白PTGS2和4-HNE,导致GPX4失活,出现铁依赖性的脂质过氧化。Ferrostatin-1可抑制整个进程,从而减少Eca-109细胞的铁死亡。结论β-榄香铜酸可通过铁死亡通路抑制人食管癌细胞Eca-109,有望成为潜在抗食管癌的药物。Objective To explore the effect ofβ-Elemenic acid on the proliferation,migration and invasion of human esophageal cancer cells Eca-109,as well as its possible mechanism.Methods Eca-109 cells were treated with different concentrations ofβ-Elemenic acid(0,20,40,60μmol/L),and the ferrostatin-1 inhibitor Ferrostatin-1(Fer-1)was used in the 60μmol/L concentration group for intervention.Firstly,MTT assay and plate colony formation assay were performed to detect the proliferation activity of Eca-109,scratch assay,transwell migration assay and transwell invasion assay were performed to detect the effect ofβ-Elephanic acid on the migration and invasion of Eca-109 cells.After that,Fer-1 was used in the 60μmol/L group to explore its mechanism.The contents of Fe^(2+),ROS,lipid ROS,GSH,and MDA in Eca-109 cells were detected by the respective kits,and the protein expressions of GPX4,PTGS2,and 4-HNE in Eca-109 cells were detected by Western blot.Resultsβ-Elemenic acid can dose-dependently inhibit the cell proliferation,colony formation,migration and invasion of Eca-109.β-Elemoceric acid can dose-dependently activate the ferroptosis pathway proteins PTGS2 and 4-HNE in Eca-109,resulting in the inactivation of GPX4 and iron-dependent lipid peroxidation of Eca-109.Ferrostatin-1 can inhibit the entire process,thereby reducing ferroptosis in Eca-109 cells.Conclusionβ-Elemocupric acid can inhibit human esophageal cancer cell Eca-109 through the ferroptosis pathway,and it is expected to become a potential anti-esophageal cancer drug.

关 键 词：β-榄香铜酸 食管癌 ECA-109 铁死亡 Ferrostatin-1 脂质过氧化 

分 类 号：R965[医药卫生—药理学]