Characteristics of traumatic brain injury models:from macroscopic blood flow changes to microscopic mitochondrial changes  

作　　者：Ding-Ding Yang Xiang-Dong Wan An-Di Chen Zi-Qian Yan Yi-Fan Lu Jun-Chen Liu Ya-Zhou Wang Jing Wang Yan Zhao Sheng-Xi Wu Guo-Hong Cai 

机构地区：[1]Department of Neurobiology,School of Basic Medicine,Forth Military Medical University,Xi’an,Shaanxi Province,China [2]Hebei Medical University,Shijiazhuang,Hebei Province,China [3]College of Life Sciences&Research Center for Resource Peptide Drugs,Yan’an University,Yan’an,Shaanxi Province,China [4]Department of Nuclear Medicine,First Affiliated Hospital of Forth Military Medical University,Xi’an,Shaanxi Province,China [5]Department of Gastroenterology,First Affiliated Hospital of Xi’an Jiaotong University,Xi’an,Shaanxi Province,China

出　　处：《Neural Regeneration Research》2023年第10期2268-2277,共10页中国神经再生研究（英文版）

基　　金：supported by grants from the National Science and Technology Innovation 2030 Grant of China,No.2021ZD0201005(to SXW);Natural Science Foundation of China,Nos.81900489(to YZ),82101294(to GHC),81730035(to SXW);Natural Science Foundation of Shaanxi Province,No.2022JM-456(to YZ);Shaanxi Provincial Key Research and Development Program,Nos.2022SF-011(to GHC),2022ZDLSF01-02(to YZW)。

摘　　要：Controlled cortical impingement is a widely accepted method to induce traumatic brain injury to establish a traumatic brain injury animal model.A strike depth of 1 mm at a certain speed is recommended for a moderate brain injury and a depth of>2 mm is used to induce severe brain injury.However,the different effects and underlying mechanisms of these two model types have not been proven.This study investigated the changes in cerebral blood flow,differences in the degree of cortical damage,and differences in motor function under different injury parameters of 1 and 2 mm at injury speeds of 3,4,and 5 m/s.We also explored the functional changes and mitochondrial damage between the 1 and 2 mm groups in the acute(7 days)and chronic phases(30 days).The results showed that the cerebral blood flow in the injured area of the 1 mm group was significantly increased,and swelling and bulging of brain tissue,increased vascular permeability,and large-scale exudation occurred.In the 2 mm group,the main pathological changes were decreased cerebral blood flow,brain tissue loss,and cerebral vasospasm occlusion in the injured area.Substantial motor and cognitive impairments were found on day 7 after injury in the 2 mm group;at 30 days after injury,the motor function of the 2 mm group mice recovered significantly while cognitive impairment persisted.Transcriptome sequencing showed that compared with the 1 mm group,the 2 mm group expressed more ferroptosis-related genes.Morphological changes of mitochondria in the two groups on days 7 and 30 using transmission electron microscopy revealed that on day 7,the mitochondria in both groups shrank and the vacuoles became larger;on day 30,the mitochondria in the 1 mm group became larger,and the vacuoles in the 2 mm group remained enlarged.By analyzing the proportion of mitochondrial subgroups in different groups,we found that the model mice had different patterns of mitochondrial composition at different time periods,suggesting that the difference in the degree of damage among traumatic brain

关 键 词：cerebral blood flow cognitive impairments controlled cortical impingement ferroptosis mitochondrial dysfunction motor impairments mouse model traumatic brain injury 

分 类 号：R651.15[医药卫生—外科学]