采用HPLC法测定盐生肉苁蓉中苯乙醇苷类含量  

Determination of phenylethanol glycosides in Cistanche salsa by HPLC

作　　者：杨雯 石妍[2] YANG Wen;SHI Yan(Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010000,China;The First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014000,China)

机构地区：[1]内蒙古医科大学附属医院,内蒙古呼和浩特010000 [2]内蒙古科技大学包头医学院第一附属医院,内蒙古包头014000

出　　处：《北方药学》2022年第12期4-8,共5页Journal of North Pharmacy

基　　金：内蒙古自治区科技计划项目(2019GG132)。

摘　　要：本文以盐生肉苁蓉中的苯乙醇类含量为评价指标,通过单因素分析最终确定一套盐生肉苁蓉中苯乙醇苷类的提取以及测定方法,最终确定以70%甲醇溶液作为提取溶剂,250W、频率40kHz下超声提取30min,该方法重现性好、准确度高。通过建立盐生肉苁蓉中苯乙醇苷类的含量测定方法,可以为全面的控制和评价盐生肉苁蓉的质量提供科学依据。Objective:In this paper,a set of methods for the extraction and determination of phenylethanol glycosides in Cistanche salsa was finally determined through single factor investigation,with the content of phenylethanol in Cistanche salsa as the evaluation index,Finally,70%methanol solution was used as the extraction solvent,and the ultrasonic extraction was carried out at 250 W and 40 kHz for 30 min.This method has good reproducibility and high accuracy.The establishment of a method for the determination of phenylethanol glycosides in Cistanche salsa can provide a scientific basis for the comprehensive control and evaluation of the quality of Cistanche salsa.

关 键 词：盐生肉苁蓉 苯乙醇苷类 含量测定 

分 类 号：R927.2[医药卫生—药学]