基于生产工艺管理的生物工程综合性实验设计  

Engineering and process management-based design of Comprehensive Biotechnology Experiment course

作　　者：宫长斌[1] 曹飞[1] 贾红华[1] 陈可泉[1] 李干禄[1] 何冰芳[1] GONG Changbin;CAO Fei;JIA Honghua;CHEN Kequan;LI Ganlu;HE Bingfang(Biochemical Engineering Experimental Teaching Center,Nanjing Technology University,Nanjing 210018,Jiangsu,China)

机构地区：[1]南京工业大学生物化学工程实验教学示范中心,江苏南京210018

出　　处：《生物工程学报》2023年第2期769-779,共11页Chinese Journal of Biotechnology

基　　金：国家一流本科专业和江苏省品牌专业建设工程;国家重点研发计划(2019YFA0905200)。

摘　　要：生物工程综合性实验课程以企业人才需求为导向,解决实际生产过程中的复杂工程问题为教学目标,利用两步酶转化法制备L-天冬氨酸和L-丙氨酸的工艺路线,结合生物工程专业生产工艺管理的特点,借鉴生产企业现场管理经验,实施四班三运转的实验运行方式。成绩考核加入交接班总结评价与团队协作评价,该课程设置了包含多门专业核心课程原理、方法与实验技术和企业生产管理模式的新型生物工程综合性实验教学内容,并通过教学实践,持续改进,形成完整的实验教学过程与考核机制。生物工程综合性实验课程取得了良好的教学效果,促进了生物工程专业实验教学的发展。Based on the demand of enterprise talents and the characteristics of manufacturing process management in biotechnology,in order to make the students acquire the ability to solve complex engineering problems in the production process,we developed a“Comprehensive Biotechnology Experiment”course,where two-step enzymatic production of L-aspartate and L-alanine were the key processes.In this course,we drew lessons from the site management of the production enterprise,performed the experimental operation mode of four shifts and three operations.The content of this course includes principles,methods and experimental techniques of several core curricula and the site management mode of enterprises.As to the evaluation,the summary of the experimental staff’s handover records and the content of teamwork were examined and scored.Through teaching practice and continuous improvement,we developed a complete experimental teaching process and assessment mechanism.Overall,the Comprehensive Biotechnology Experiment course achieved good teaching effect,which may serve as a reference to promote the development of experimental teaching of biotechnology.

关 键 词：生物工程 工艺设计 工程管理 综合性实验 

分 类 号：Q81-4[生物学—生物工程] G642.423[文化科学—高等教育学]