清肺排毒汤水煎液中4种不同水溶解度有效成分的转移率研究  

Study on the Transfer Rate of Four Effective Components with Different Water Solubility in Qingfei Paidu Decoction

作　　者：闫斌 陈树和[1,2,3] 段雪云 陈洪燕[1,2,3] 朱田密 段雨晴[1,2,3] YAN Bin;CHEN Shuhe;DUAN Xueyun;CHEN Hongyan;ZHU Tianmi;DUAN Yuqing(Department of Pharmacy,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China;Department of Pharmacy,Affiliated Hospital of Hubei University of Chinese Medicine,Wuhan 430061,China;Institute of Traditional Chinese Medicine,Hubei Province Academy of Traditional Chinese Medicine,Wuhan 430074,China)

机构地区：[1]湖北省中医院药事部,武汉430061 [2]湖北中医药大学附属医院药事部,武汉430061 [3]湖北省中医药研究院中药研究所,武汉430074

出　　处：《医药导报》2023年第3期346-352,共7页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(2020CFB438);国家重点研发计划-中医药现代化研究重点专项(2017YFC1703400)。

摘　　要：目的测定清肺排毒汤水煎液中4种不同水溶解度有效成分的转移率,为其质量控制研究和临床应用提供依据。方法建立3种高效液相色谱(HPLC)条件测定编号S1~S10清肺排毒汤水煎液中盐酸麻黄碱和盐酸伪麻黄碱、苦杏仁苷、黄芩苷含量,并测定这4种成分在所归属饮片中的含量,计算4种成分由饮片至水煎液的转移率,从4种成分中筛选水煎液质量控制的指标成分。结果盐酸麻黄碱和盐酸伪麻黄碱转移率为40.99%~52.01%;苦杏仁苷转移率为13.78%~27.68%;黄芩苷转移率为2.67%~6.35%,4种成分转移率差异有统计学意义(P<0.05),水煎液中4种成分含量均在安全有效范围内,盐酸麻黄碱和盐酸伪麻黄碱可作为其质量控制指标成分。结论该研究可为清肺排毒汤水煎液的质量控制和临床应用提供参考依据。Objective To study the transfer rates of four effective components with different water solubility in Qingfei Paidu decoction(QFPDD),and to provide the basis for its quality control research and clinical application.Methods Three kinds of high-performance liquid chromatography(HPLC)conditions were established to determine the contents of ephedrine hydrochloride,pseudoephedrine hydrochloride,amygdalin and baicalin in QFPDD with number S1-S10,and the contents of these four components in the decoction pieces were determined.The transfer rate of four components from decoction pieces to QFPDD was calculated,and the decoction quality control index components were screened in these four components.Results The transfer rate of ephedrine hydrochloride and pseudoephedrine hydrochloride from decoction pieces to QFPDD was 40.99%-52.01%.The transfer rate of amygdalin from decoction pieces to QFPDD was 13.78%-27.68%.The transfer rate of baicalin from decoction pieces to QFPDD was 2.67%-6.35%.The transfer rate of the four components was statistically significant(P<0.05).The contents of the four components in QFPPD were within the safe and effective range.Ephedrine hydrochloride and pseudoephedrine hydrochloride can be used as indicators for quality control of QFPDD.Conclusion This study provided a reference for the quality control and clinical application of QFPDD.

关 键 词：清肺排毒汤 水煎液 水溶解度 含量测定 转移率 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]