ASH2L在幽门螺旋杆菌感染的人胃癌细胞系中的表达及临床意义  

Expression and clinical significance of ASH2L in human gastric carcinoma cell lines infected with Helicobacter pylori

作　　者：谢晓婷 张雷 张莉 董凤 刘颖 李光华[1] 杨兴 XIE Xiaoting;ZHANG Lei;ZHANG Li;DONG Feng;LIU Ying;LI Guanghua;YANG Xing(Institute of Pathogens and Vectors,Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention,Dali University,Dali 671000;Department of Clinical Laboratory,the Eastern Division,First Hospitipal of Jilin University,Changchun 130000,China)

机构地区：[1]大理大学病原与媒介生物研究所云南省自然疫源性疾病防控技术重点实验室,云南大理671000 [2]吉林大学第一医院二部检验科,吉林长春130000

出　　处：《基础医学与临床》2023年第3期367-373,共7页Basic and Clinical Medicine

基　　金：国家自然科学基金(81560096);大理大学临床分子免疫学创新团队项目(ZKLX2019105)。

摘　　要：目的探索果蝇蛋白Ash2的类似物(ASH2L)在幽门螺旋杆菌(Hp)感染的人胃癌细胞系中的表达水平及临床意义。方法利用TIMER2.0、UALCAN等生物信息软件分析ASH2L在多种肿瘤中的表达水平、与胃腺癌患者临床病理特征及与预后的关系。用Hp分别感染人胃上皮细胞系GES、人胃腺癌细胞系SGC-7901和AGS,以RT-qPCR、Western blot检测ASH2L在Hp感染前后的表达水平。结果生物信息分析显示ASH2L在15种肿瘤中存在差异表达,且其高表达于胃腺癌组织中;在胃癌患者中,ASH2L高表达者总生存期(OS)和后进展生存期(PPS)低于低表达者(P<0.05);不同年龄组、不同种族、不同病理分级的胃腺癌患者中ASH2L的表达存在一定的差异,但与男女性别无关;Hp感染的3种人胃上皮细胞系中ASH2L表达水平均显著上调(P<0.05)。结论ASH2L在Hp感染引发的胃癌的发生发展中可能发挥重要作用,其表达水平与胃癌患者各种临床病理特征及预后密切相关,可望作为判断预后的分子标志物。Objective To explore the expression and clinical significance of Drosophila protein Ash2 analogue(ASH2L)in gastric carcinoma cell lines infected with Helicobacter pylori(Hp).Methods The expression of ASH2L in various tumors,the relationship between ASH2L and pathological characteristics of stomach adenocarcinoma and the prognosis of the patients were analyzed by TIMER2.0,UALCAN and other software.Human gastric mucosal epithelial cell line GES,gastric carcinoma cell lines SGC-7901 and AGS were infected with Hp and the expression of ASH2L before and after Hp infection was detected by RT-qPCR and Western blot.Results The analysisshowed that ASH2L was differentially expressed in 15 kinds of tumors and was highly expressed in stomach adenocarcinoma.The overall survival(OS)and post-progression survival(PPS)of patients with high ASH2L expression were lower than those with low ASH2L expression(P<0.05).There were some differences in the expression of ASH2L in patients with stomach adenocarcinoma in different age groups,different races and different pathological grades,but not related to gender.The expression of ASH2L was significantly up-regulated in three human gastric epithelial cell lines infected with Hp(P<0.05).Conclusions ASH2L may play an important role in tumorigenesis of gastric carcinoma caused by Hp infection,and its expression level is closely related to various pathological features and prognosis of the patients,and it is expected to be a molecular marker for prognosis.

关 键 词：幽门螺旋杆菌 胃癌 ASH2L 临床预后 

分 类 号：R378[医药卫生—病原生物学]