全杜仲胶囊对自发性高血压大鼠肠壁微血流灌注的影响  

Effects of Quanduzhong Jiaonang on microcirculatory blood perfusion of intestinal wall in spontaneously hypertensive rats

作　　者：王琴[1] 张晓艳[1] 刘雪婷 李炳蔚[1] 李宏伟[1] 杨义力 韩建群[1] WANG Qin;ZHANG Xiaoyan;LIU Xueting;Li Bingwei;LI Hongwei;YANG Yili;HAN Jianqun(Institute of Microcirculation,CAMS&PUMC,Beijing 100005;China Regional Research Center(Taizhou),International Center for Genetic Engineering and Biotechnology,Taizhou 225300,China)

机构地区：[1]中国医学科学院北京协和医学院微循环研究所,北京100005 [2]国际遗传工程和生物技术中心中国区域研究中心(泰州),江苏泰州225300

出　　处：《基础医学与临床》2023年第3期386-392,共7页Basic and Clinical Medicine

基　　金：国家自然科学基金(81973358);中国医学科学院微循环研究所基础科研项目(WXH2019002)。

摘　　要：目的 探讨全杜仲胶囊(QDZJN)和松脂醇二葡萄糖苷(PDG)对自发性高血压大鼠(SHRs)肠壁微循环血流灌注及其频谱特征的影响。方法 将SHRs随机分为SHR,SHR+QDZJN,SHR+PDG(L),SHR+PDG (M)和SHR+PDG (H)组。对照组为Wistar-kyoto(WKY)鼠。各组分别接受QDZJN或纯净水(对照组和SHR组)灌胃2个月。手术暴露大鼠小肠,激光多普勒血流探测仪(LDF)和血流成像仪(LDPI)检测并比较各组大鼠肠壁微血流灌注水平。小波分析比较各组肠壁内皮源性频段幅值。结果 与对照组相比,SHRs肠壁血流灌注水平显著降低(P<0.001)。QDZJN和各剂量PDG治疗2个月后,SHRs肠壁血流灌注水平显著升高(P<0.001)。SHRs组肠壁微血流灌注一氧化氮(NO)依赖和非依赖性内皮细胞源频段振幅均显著低于对照组(P<0.01)。QDZJN和PDG治疗可显著提高内皮细胞源频段幅值(NO依赖:P<0.0001;NO非依赖:P<0.01)。结论 QDZJN和PDG治疗2个月可显著改善SHRs肠壁的微血流动力学和内皮功能障碍。Objective To investigate the effects of Quanduzhong Jiaonang(QDZJN) and pinoresinol diglucoside(PDG) on microcirculatory blood perfusion in intestinal wall and its spectral characteristics in spontaneously hypertensive rats(SHRs).Methods SHRs were randomly divided into SHR,SHR+QDZJN,SHR+PDG(L),SHR+PDG(M) and SHR+PDG(H) groups with 6 in each group.The control group consisted of 6 WKY rats.All treatment groups were given intragastric administration of QDZJN,PDG,or pure water.After 2 month continuous administration,rats’ intestines were exposed by surgery.The wall microcirculatory blood perfusion of was measured by laser Doppler flowmetry(LDF) and laser Doppler perfusion imaging(LDPI).Wavelet transform analysis was performed,and the amplitudes of NO-independent endothelial and NO-dependent endothelial oscillators were compared among six groups.Results Compared with the control group,blood perfusion level of intestine wall was significantlydecreased in SHRs group(P<0.001).After two months of treatment with QDZJN and PDG,the blood perfusion level of SHRs was significantly elevated(P<0.001).The amplitudes of NO-dependent and-independent endothelial activity in SHRs group were significantly lower than those in the control group(both P<0.01).QDZJN and PDG significantly enhanced the amplitude of NO-dependent and-independent endothelial activity of intestine wall perfusion in SHRs(P<0.0001 and P<0.01).Conclusions Treatment with QDZJN and PDG for two months may significantly improve the microhemodynamics and endothelial function of intestine wall in SHRs.

关 键 词：杜仲 肠壁 微循环 血流灌注 自发性高血压大鼠 

分 类 号：R331.3[医药卫生—人体生理学] R544.1[医药卫生—基础医学]