葛根素减轻KKAy小鼠血管内皮细胞炎性反应  

Puerarin attenuates the inflammatory response of vascular endothelial cells in KKAy mice

作　　者：邹洁 张德德 陶毅[1] 赵静[1] 王琼[1] 徐乃佳[1] ZOU Jie;ZHANG Dede;TAO Yi;ZHAO Jing;WANG Qiong;XU Naijia(Department of Endocrinology,Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430050,China)

机构地区：[1]武汉市中医医院内分泌科,湖北武汉430050

出　　处：《基础医学与临床》2023年第3期433-437,共5页Basic and Clinical Medicine

基　　金：武汉市卫生和计划生育委员会项目(WZ18D12)。

摘　　要：目的 探究葛根素(PUR)对KKAy小鼠(2型糖尿病模型小鼠)血管内皮细胞炎性反应的干预机制。方法 将C57BL/6J小鼠作为对照组,KKAy小鼠随机分为糖尿病组、二甲双胍(MH)及葛根素(PUR)组,每组10只。检测各组糖代谢指标,ELISA检测肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、转化生长因子-β1(TGF-β1)、白介素-1(IL-1)、血管内皮生长因子(VEGF)含量变化;TUNEL法检测血管组织细胞凋亡;RT-qPCR及Western blot检测血管细胞黏附分子-1(VCAM-1)、细胞间黏附分子-1(ICAM-1)mRNA及蛋白表达。结果 与对照组相比,糖尿病组糖代谢指标明显升高,细胞凋亡增加;血清中TNF-α、IFN-γ、TGF-β1、IL-1、VEGF含量明显升高(P<0.05),血管组织中VCAM-1和ICAM-1 mRNA及蛋白表达明显增加(P<0.05)。与糖尿病组相比,MH组及PUR组糖代谢指标明显改善,细胞凋亡明显减轻;TNF-α、IFN-γ、TGF-β1、IL-1、VEGF含量明显降低(P<0.05);VCAM-1和ICAM-1 mRNA及蛋白表达明显改善(P<0.05)。结论 PUR能够有效降低KKAy小鼠血管内皮炎性因子表达,降低VCAM-1和ICAM-1黏附分子表达,改善糖尿病小鼠血管内皮损伤。Objective To explore the intervention mechanism of puerarin(PUR) on the inflammatory response of vascular endothelial cells with KKAy mouse models.Methods C57bL/6j mice were used as the control group.KKAy mice were randomly divided into diabetes group,metformin hydrochloride(MH)and puerarin(PUR) group with 10 in each.The indexes of glucose metabolism in each group were recorded.The contents of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),transforming growth factor-β1(TGF-β1),interleukin-1(IL-1) and vascular endothelial growth factor(VEGF) were detected by ELISA;TUNEL method was used to detect apoptosis in vascular tissue;mRNA and protein expression of vascular cell adhesion molecule-1(VCAM-1) and intercellular adhesion molecule-1(ICAM-1) were detected by RT-qPCR and Western blot.Results Compared with the control group,the indexes of glucose metabolism and apoptosis in diabetes group increased significantly;The contents ofTNF-α,IFN-γ,TGF-β1,IL-1 and VEGF in serum changed significantly(P<0.05) and the mRNA and protein expressions of VCAM-1 and ICAM-1 in vascular tissue increased significantly(P<0.05).Compared with diabetes group,MH group and PUR group significantly improved glucose metabolism and cell apoptosis;TNF-α,IFN-γ,TGF-β1,IL-1 and VEGF decreased significantly(P<0.05);The mRNA and protein expressions of VCAM-1 and ICAM-1 were all significantly improved(P<0.05).Conclusions PUR can effectively inhibit the expression of vascular endothelial inflammatory factors,VCAM-1 and ICAM-1 adhesion molecules in KKAy mice,and alleviate the vascular endothelial injury found in diabetes mice.

关 键 词：糖尿病 葛根素 血管内皮损伤 血管细胞黏附分子-1(VCAM-1) 细胞间黏附分子-1(ICAM-1) 

分 类 号：R587.1[医药卫生—内分泌]