磁示踪法在硬膜外动脉贴敷术加速丘脑组织间液引流机制研究中的应用  

Mechanism of epidural artery implantation in accelerating the drainage of thalamic interstitial fluid by using tracer-based MRI

作　　者：周洁 连婧阁 任秋实 王卉 孔东生[5] 王伟 刘会坡 李宏峰 程晋 韩鸿宾[1,2,3] ZHOU Jie;LIAN Jingge;REN Qiushi;WANG Hui;KONG Dongsheng;WANG Wei;LIU Huipo;LI Hongfeng;CHENG Jin;HAN Hongbin(Institute of Medical Technology,Peking University Health Science Center,Beijing 100191,China;Department of Radiology,Peking University Third Hospital,Beijing 100191,China;Beijing Key Lab of Magnetic Resonance Imaging Device and Technique,Beijing 100191,China;Shenzhen Research Institute of Peking University,Shenzhen 518055,China;Department of Neurosurgery,First Medical Center,General Hospital of Chinese PLA,Beijing 100191,China;Department of Radiology,Beijing Rehabilitation Hospital,Capital Medical University,Beijing 100191,China;School of Mathematical Science,Fudan University,Shanghai 200433,China)

机构地区：[1]北京大学医学部医学技术研究院,北京100191 [2]北京大学第三医院放射科,北京100191 [3]北京市磁共振成像设备与技术重点实验室,北京100191 [4]北京大学深圳研究生院,深圳518055 [5]解放军总医院第一医学中心神经外科,北京100191 [6]首都医科大学附属北京康复医院放射科,北京100191 [7]复旦大学数学科学学院,上海200433

出　　处：《磁共振成像》2023年第2期97-102,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金(编号:12126601);深圳市海外高层次人才“孔雀计划”(编号:KQTD20180412181221912)。

摘　　要：目的为了研究硬膜外动脉贴敷术(epidural arterial implantation,EAI)对脑组织间液(interstitial fluid,ISF)流动的影响及其机制。材料与方法将30只240~280 g雄性SD(Sprague Dawley)大鼠随机分成空白对照组、EAI组(术后7 d、14 d)、EAI对侧测量组(术后7 d、14 d)5组,每组均为6只。用磁示踪法对丘脑区细胞外间隙(extracellular space,ECS)内ISF引流进行探测,用扩散系数分布图(diffusion of extracellular space-mapping,D_(ECS)-mapping)技术研究ISF引流改变的潜在机制,获取宏观ISF引流分布指标:半衰期T_(1/2),以及ECS结构参数:扩散速率D^(*)、迂曲度λ、容积占比α。同时,应用激光多普勒血流仪观察大鼠顶叶皮层局部脑血流变化,并应用旷场实验和新物体识别实验等方法测试大鼠的运动和认知能力。结果磁示踪法和D_(ECS)-mapping分析显示,EAI7、EAI14组的半衰期较其余组下降(P<0.01),表示同侧丘脑区ISF流动速度加快;EAI7、EAI14组的扩散速率、容积占比较其余组上升(P<0.05),迂曲度较其余组下降(P<0.05),代表同侧丘脑区ECS构象变化导致分子扩散运动加快。空白对照组、EAI14组的脑血流量未见差异(P>0.05),表示该手术短期内未改变大鼠局部脑血流量。大鼠行为学检测各组指标未见差异(P>0.05),表明该手术未损害大鼠运动、认知能力。结论EAI可以通过改变大鼠同侧丘脑区ECS微观结构,促进ISF流动,是一种稳定、安全、有效的脑ECS主动调控手段。Objective:To investigate the effect of epidural arterial implantation(EAI)on the drainage of interstitial fluid(ISF)in rat thalamus and its mechanism.Materials and Methods:Thirty 240-280 g male SD rats were randomly divided into control group,EAI group and EAI contralateral measurement group.The latter two groups were randomly divided into two subgroups on the 7th and 14th day after operation,with six rats in each group.The ISF drainage in thalamus was detected by tracer-based MRI and using diffusion of extracellular space-mapping(D_(ECS)-mapping)techniques to study the structure of extracellular space(ECS).The macroscopic distribution index of ISF drainage:half-life time(T_(1/2)),and the structural parameters of ECS:diffusion rate(D^(*)),tortuosity(λ),volume fraction(α)were obtained.The changes of regional cerebral blood flow in the parietal cortex of rats were observed by laser Doppler flow meter under the guidance of stereotactic instrument.Motor and cognitive performance was quantified with open filed test and novel recognition test at baseline and 7,14 days after EAI.Results:Tracer-based MRI and D_(ECS)-mapping analysis showed that the half-life time of EAI7 and EAI14 groups was lower than that of the other groups(P<0.01);the diffusion rate and volume fraction of EAI7 and EAI14 groups increased compared with other groups(P<0.05),and the tortuosity decreased compared with other groups(P<0.05),indicating that the molecular diffusion movement in ECS was accelerated and the structure of ECS was changed.There was no difference in cerebral blood flow between the control group and EAI14 group(P>0.05),indicating that the operation did not change the local cerebral blood flow of rats in a short time.There was no difference in the indexes of animal behavior test among the groups(P>0.05),indicating that the operation did not damage the motor and cognitive abilities of rats.Conclusions:EAI changed the microstructure of ECS in the ipsilateral thalamus of rats and promoted the drainage of ISF,which was a stable,safe and

关 键 词：硬膜外动脉贴敷术 脑 细胞外间隙 组织间液 磁示踪 磁共振成像 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]