Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line‘AT7–4'  

作　　者：Hui Li Aixia Gu Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei,Collaborative Innovation Center of Vegetable Industry in Hebei,Baoding,Hebei 071000,China [2]College of Horticulture,Hebei Agricultural University,Baoding,Hebei 071000,China [3]College of Life Science,Hebei Agricultural University,Baoding,Hebei 071000,China

出　　处：《Horticultural Plant Journal》2023年第1期89-97,共9页园艺学报（英文版）

基　　金：supported by the State Key Program of National Natural Science Foundation of China(Grant Nos.31930098,31772324);Hebei Provincial Natural Science Fund for Distinguished Young(Grant No.C2020204063);Natural Science Foundation and basic research project in Hebei Province(Grant No.18966925D);the Innovative Research Group Project of Hebei Natural Science Foundation(Grant No.C2020204111);the Agricultural Science and Technology Innovation Program of CAAS(Grant No.CAASXTCX2019025);the National Natural Science Foundation of China(Grant No.31672151);the Science and Technology Support Program of Hebei(Grant No.16226304D-2);Science and Technology Research Project of Universities in Hebei Province(BJ2019020);the International Science and Technology Cooperation base Special Project of Hebei(Grant No.20592901D)。

摘　　要：Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes.Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage.In this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring.Consequently,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week vernalization.Based on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed.This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.

关 键 词：Chinese cabbage Translocation line Alien gene Molecular marker Late flowering 

分 类 号：S634.1[农业科学—蔬菜学]