Interaction between MAPKs and MKPs in hexaploid chrysanthemum illuminates functional paralogue diversification in polyploids  

作　　者：Qi Yu Luyao Zhang Daojin Sun Yueheng Hu Peiling Li Xue Zhang Lian Ding Lijie Zhou Zhiyong Guan Weimin Fang Fadi Chen Aiping Song 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Key Laboratory of Landscaping,Ministry of Agriculture and Rural Affairs,College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Henan Key Laboratory of Tea Comprehensive Utilization in South Henan,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000,China

出　　处：《Horticultural Plant Journal》2023年第1期158-168,共11页园艺学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant Nos.31870694,31870279,32002083);the Natural Science Fund of Jiangsu Province(Grant No.BK20190076);the National Key R&D Program of China(Grant No.2018YFD1000403);the Foundation of Central Laboratory of Xinyang Agriculture and Forestry University(Grant No.FCL202002);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institution。

摘　　要：Mitogen-activated protein kinases(MAPKs,also known as MPKs)regulate diverse cellular and physiological functions,and dual-specificity MAPK phosphatases(MKPs)modulate MAPK signalling through MAPK dephosphorylation and inactivation.Due to lacking of overall understanding for the regulatory networks between Chrysanthemum morifolium MKPs(CmMKPs)and C.morifolium MAPKs(CmMPKs),we systematically studied the interactions between four groups of CmMPKs and eight identified CmMKPs in chrysanthemum and found that the interaction between the specific CmMKP and the specific CmMPK differed from those in other plants.Furthermore,the expression of CmMKP1 and CmMKP1-LIKE1showed opposite trends during the development of chrysanthemum flower buds under salt treatment and Alternaria alternata inoculation,but these genes could interact with the same CmMPKs,providing insight into the subfunctionalization of paralogues.Amino acid variations(M87V,T277P and V6L)in dual-specificity protein phosphatases(DsPTP1)-LIKE1/2/3 changed the interactions of these proteins with the four CmMPK groups in chrysanthemum,providing evidence for the de/neofunctionalization of paralogues in polyploids,suggesting that we can identify the key functional sites of proteins by studying polyploid paralogues.

关 键 词：CHRYSANTHEMUM Evolution MAPK MKP Protein structure Yeast two-hybrid 

分 类 号：S682.11[农业科学—观赏园艺]