单细胞转录组测序在多发性大动脉炎肾动脉病变中的初步探索  

Preliminary Results of Single-cell Transcriptome Sequencing in Renal Arterial Lesions of Takayasu Arteritis

作　　者：高擎 吴志远 李海洋[1] 李拥军[2] GAO Qing;WU Zhiyuan;LI Haiyang;LI Yongjun(Department of Cardiovascular Surgery,Beijing Anzhen Hospital,Capital Medical University,Beijing 100029,China;Department of Vascular Surgery,Beijing Hospital,Beijing 100730,China)

机构地区：[1]首都医科大学附属北京安贞医院心血管外科,北京100029 [2]北京医院血管外科,北京100730

出　　处：《中国医学科学院学报》2023年第1期80-87,共8页Acta Academiae Medicinae Sinicae

基　　金：中央高水平医院临床科研业务费(BJ-2021-196)。

摘　　要：目的探索单细胞转录组测序(scRNA-seq)在多发性大动脉炎(TA)肾动脉病变中的初步应用。方法纳入2例北京医院血管外科进行肾动脉手术搭桥治疗的TA患者,将其肾动脉组织样本分别采用GEXSCOPE试剂盒和自配消化液2种酶解方式,并进行scRNA-seq和生物信息学分析。结果共获取2920个细胞纳入下游分析。经过无偏聚类分析后,确定2种内皮细胞、2种平滑肌细胞、1种成纤维细胞、2种单核巨噬细胞、1种T细胞及1种未定义的细胞。其中,2种平滑肌细胞主要表现为收缩型及分泌型。scRNA-seq分析结果显示试剂盒酶解法获取的细胞以内皮细胞(57.46%)为主,免疫细胞(13.21%)较少,而自配消化液酶解法获取的细胞以免疫细胞(34.64%)为主。结论scRNA-seq可用于区分TA患者病变血管的细胞异质性,不同酶解方式会影响获取细胞的种类与数量。Objective To explore the preliminary application of single-cell RNA sequencing(scRNA-seq)in the renal arterial lesions in Takayasu arteritis(TA)patients.Methods This study included 2 TA patients with renal artery stenosis treated by bypass surgery in the Department of Vascular Surgery,Beijing Hospital.The obtained 2 renal artery samples were digested with two different protocols(GEXSCOPE kit and self-made digestion liquid)before scRNA-seq and bioinformatics analysis.Results A total of 2920 cells were obtained for further analysis.After unbiased cluster analysis,2 endothelial cell subsets,2 smooth muscle cell subsets,1 fibroblast subset,2 mononuclear macrophage subsets,1 T cell subset,and 1 undefined cell subset were identified.Among them,the two subsets of smooth muscle cells were contractile and secretory,respectively.The results of scRNA-seq indicated that enzymatic hydrolysis with GEXSCOPE kit produced a large number of endothelial cells(57.46%)and a small number of immune cells(13.21%).However,immune cells(34.64%)were dominant in the cells obtained by enzymatic hydrolysis with self-made digestive liquid.Conclusion scRNA-seq can be employed to explore the cellular heterogeneity of diseased vessels in TA patients.Different enzymatic digestion protocols may impact the proportion of different cells.

关 键 词：大动脉炎 单细胞测序 细胞异质性 内皮细胞 平滑肌细胞 

分 类 号：R654.3[医药卫生—外科学]