棉花色素腺体发育相关基因GhERF105-like的克隆与表达分析  

Cloning and Expression Analysis of a Cotton Pigment Gland Formation Related Gene GhERF105-like

作　　者：张曦 钱玉源 王广恩[1] 刘祎[1] 权月伟[2] 崔淑芳[1] 米换房 李俊兰[1] ZHANG Xi;QIAN Yuyuan;WANG Guang′en;LIU Yi;QUAN Yuewei;CUI Shufang;MI Huanfang;LI Junlan(Institute of Cotton,Hebei Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Cotton in Huanghuaihai Semiarid Area,Ministry of Agriculture,National Cotton Improvement Center Hebei Branch,Shijiazhuang 050051,China;Handan Academy of Agricultural Sciences,Handan 056001,China)

机构地区：[1]河北省农林科学院棉花研究所,农业部黄淮海半干旱区棉花生物学与遗传育种重点实验室,国家棉花改良中心河北分中心,河北石家庄050051 [2]邯郸市农业科学院,河北邯郸056001

出　　处：《华北农学报》2022年第6期34-41,共8页Acta Agriculturae Boreali-Sinica

基　　金：河北省农林科学院科技创新专项(2022KJCXZX-MHS-4);河北省现代农业产业技术体系项目(HBCT2018040207)。

摘　　要：为了探析GhERF105-like在棉花色素腺体发育或棉酚代谢中的功能,利用RT-PCR技术克隆了中棉所12的GhERF105-like基因,并进行了生物信息学分析及表达模式分析。结果显示,GhERF105-like编码区为711 bp,编码236个氨基酸。GhERF105-like蛋白分子质量为26.3 ku,理论等电点pI为7.72;二级结构预测存在22.88%的α-螺旋、13.14%的延伸链、3.81%的β-转角和60.17%的无规则卷曲。GhERF105-like在第91-155位氨基酸处存在1个AP2结构域,预测有2个苏氨酸、1个酪氨酸和1个丝氨酸磷酸化位点位于AP2结构域内。系统进化分析表明,GhERF105-like及其同源蛋白基序组成在不同物种中保守程度较高,GhERF105-like与黄褐棉、达尔文氏棉、雷蒙德氏棉、海岛棉中同源蛋白的基序组成一致。GhERF105-like在有腺体棉中棉所12各个组织中的表达均显著或极显著高于无腺体棉中棉所12显性无腺体;GhERF105-like在不同有腺体棉品种/系叶片中的表达量不同,且均显著高于显性或隐性无腺体棉品种/系。GhERF105-like受ABA、BR诱导显著上调表达,但在MeJA、Eth、NaCl和PEG处理下显著或极显著下调表达。综上所述,GhERF105-like与棉花色素腺体发育密切相关,可能通过ABA、BR、Eth和JA通路调控腺体发育或棉酚代谢。In order to explore the function of GhERF105-like in the development of pigment glands or gossyol metabolism of cotton,the GhERF105-like gene of Zhongmiansuo 12 was cloned by RT-PCR,and the bioinformatics analysis and expression patterns were analyzed.The results showed that the coding region of GhERF105-like was 711 bp,encoding 236 amino acids.The molecular weight of GhERF105-like was 26.3 ku,and the theoretical pI was 7.72.The predicted secondary structure of GhERF105-like was 22.88%α-helix,13.14%extended strand,3.81%β-turn and 60.17%random coil.GhERF105-like had an AP2 domain at 91-155 aa.Two threonine,one tyrosine and one serine phosphorylation sites were predicted to be located in the AP2 domain.Phylogenetic analysis showed that motifs of GhERF105-like and its homologous proteins were highly conserved in different species.GhERF105-like motifs were consistent with homologous proteins in Gossypium hirsutum,G.mustelinum,G.darwinii,G.raimondii and G.barbadense.The expression of GhERF105-like in each tissue of glanded cotton Zhongmiansuo 12 was significantly or extremely significantly higher than that of dominant glandless Zhongmiansuo 12.The expression levels of GhERF105-like in leaves of different glanded cotton varieties/lines were different,and were all significantly higher than the expressions of dominant or recessive glandless cotton varieties/lines.GhERF105-like was significantly up-regulated by ABA and BR,but significantly or extremely significantly down-regulated by MeJA,Eth,NaCl and PEG treatment.In conclusion,GhERF105-like is closely related to the development of pigment glands in cotton,and may regulate gland development or gossypol metabolism through ABA,BR,Eth and JA pathways.

关 键 词：棉花 AP2/ERF转录因子 序列分析 表达分析 

分 类 号：S562[农业科学—作物学] Q78[生物学—分子生物学]