大型迪庆藏猪不同生长阶段背脂与腹脂脂质代谢差异基因及调控网络分析  

Key Genes and Regulatory Network Analysis of Lipid Metabolism Differences between Back Fat and Abdominal Fat of Large Diqing Tibetan Pigs at Different Growth Stages

作　　者：王琳 马黎[2] 张博[3] 邓俊 张浩[3] 欧阳晓芳 严达伟[1] 董新星[1] WANG Lin;MA Li;ZHANG Bo;DENG Jun;ZHANG Hao;OUYANG Xiaofang;YAN Dawei;DONG Xinxing(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Vocational and Technical College of Agriculture,Kunming 650212,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;Yunnan Province Animal Husbandry Station,Kunming 650224,China)

机构地区：[1]云南农业大学动物科学技术学院,昆明650201 [2]云南农业职业技术学院,昆明650212 [3]中国农业大学动物科学技术学院,北京100193 [4]云南省畜牧总站,昆明650224

出　　处：《畜牧兽医学报》2023年第2期520-533,共14页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：云南省乡村振兴科技专项项目(202104BI090021);云南省重大科技专项项目(202202AE090005);云南省农业联合面上项目(2021BD070001-10)。

摘　　要：旨在筛选大型迪庆藏猪不同生长阶段、不同部位脂质代谢差异的关键基因。本研究选择胎次相同、出生日期相近、体重10 kg左右的大型迪庆藏猪36头,随机分为3组,相同条件育肥,分别在平均体重达40、80和120 kg时屠宰,测定胴体性能,每组采集3头猪的背脂和腹脂进行高通量转录组测序,测序数据经拼接、比对,筛选与脂质代谢相关的显著差异基因并进行GO、KEGG分析、基因互作网络分析。结果表明,40、80和120 kg大型迪庆藏猪腹脂vs.背脂分别筛到486、765和339个差异表达显著基因,随机挑选的EGR2、SOD3等5个差异表达显著基因的qPCR结果与转录组测序结果一致,差异表达显著基因主要富集在肌肉收缩、细胞黏附、间充质细胞增殖正调节等GO条目,心肌收缩、PI3K-Akt信号通路、Hippo信号通路等KEGG通路;40 kg组EGR2、RARRES2、TMOD4和SFRP2基因位于网络核心,EGR2、RARRES2、SFRP2在腹脂上调,TMOD4下调;80 kg组THBS1、PPARA、NRIP1和LPL基因位于网络核心,4个核心基因均在腹脂上调;120 kg组HTRA1、TSHR、LRRK2、STC2、SHOX2和SOD3基因位于网络核心,LRRK2、TSHR在腹脂上调,SHOX2、SOD3、STC2、HTRA1下调。结果提示,EGR2、THBS1、TSHR等14个基因作为核心基因精细调控大型迪庆藏猪不同生长阶段背脂与腹脂脂质代谢,10~40 kg,EGR2等4个基因位于核心,促进脂肪细胞分化、增殖的基因在腹脂上调,脂肪合成的开关基因下调;40~80 kg,THBS1等4个基因位于核心,促进甘油三酯合成、胆固醇形成、脂质积累的基因在腹脂上调;80~120 kg,LRRK2等6个基因位于核心,促进甘油三酯积累、脂肪酸氧化的基因在腹脂上调,抑制脂肪分解、脂滴形成的基因下调。本试验结果可为解析地方猪不同部位脂质差异性沉积的调控机制提供基础数据,为迪庆藏猪的靶向选育提供参考。The aim of this study was to screen the key genes of lipid metabolism differences in back fat and abdominal fat of large Diqing Tibetan pigs(TPs) at different growth stages by transcriptome sequencing. In this study, 36 TPs with the same parity, birth date and weight of about 10 kg were randomly divided into 3 groups for fattening experiment under the same conditions. The pigs were slaughtered at the weight of 40, 80 and 120 kg, respectively, and carcass perfor-mance was measured. Back fat(BF) and abdominal fat(AF) of 3 pigs in each group were collected for high-throughput transcriptome sequencing. The sequencing data were spliced and compared to screen for genes with significant differences related to lipid metabolism, and then GO, KEGG analysis and gene interaction network analysis were performed. The results showed that 486, 765 and 339 differentially expressed genes(DEGs) were screened from AF vs. BF of large Diqing Tibetan pigs weighing 40, 80 and 120 kg, respectively. The qPCR results of randomly selected 5 significantly DEGs such as EGR2 and SOD3 were consistent with the transcriptome sequencing results. These DEGs were mainly enriched in GO items such as muscle contraction, cell adhesion and positive regulation of mesenchymal cell proliferation, and KEGG pathways such as cardiac muscle contraction, PI3K-Akt signaling pathway, and Hippo signaling pathway. In 40 kg group, EGR2, RARRES2, TMOD4 and SFRP2 genes were located in the network core, EGR2, RARRES2 and SFRP2 were up-regulated in abdominal fat, and TMOD4 was down-regulated. In 80 kg group, THBS1, PPARA, NRIP1 and LPL genes were located in the core of the network, and all 4 core genes were up-regulated in AF. In 120 kg group, HTRA1, TSHR, LRRK2, STC2, SHOX2 and SOD3 genes were located in the network core, LRRK2 and TSHR were up-regulated in AF, while SHOX2, SOD3, STC2 and HTRA1 were down-regulated. The results suggested that 14 core genes including EGR2, THBS1 and TSHR finely regulated the lipid metabolism of BF and AF at different growth stages of TPs

关 键 词：迪庆藏猪 脂肪差异沉积 功能基因 基因互作网络 

分 类 号：S828.2[农业科学—畜牧学]