马钱内生真菌Exserohilum rostratum次生代谢产物研究  

Investigation on secondary metabolites of endophytic fungus Exserohilum rostratum hosted in Strychnos nux-vomica

作　　者：易诚 曹海燕 陈科良 杨晨宇 刘云宝 YI Cheng;CAO Hai-yan;CHEN Ke-liang;YANG Chen-yu;LIU Yun-bao(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《中草药》2023年第1期35-40,共6页Chinese Traditional and Herbal Drugs

基　　金：中国医学科学院医学与健康科技创新工程项目(CIFMS-2022-I2M-JB-009)。

摘　　要：目的 对有毒植物马钱内生真菌Exserohilumrostratum的次生代谢产物进行研究。方法 通过硅胶柱色谱及反相HPLC等多种色谱分离方法进行分离纯化,运用高分辨质谱、核磁共振波谱、红外、紫外及圆二色谱等分析方法对所分离的化合物进行结构鉴定。采用Griess法对化合物进行抗炎活性测试。结果 从有毒植物马钱内生真菌Exserohilum rostratum的发酵产物中分离得到6个化合物,分别鉴定为exserone A(1)、(3R,4R)-4,8-二羟基-3-[(R)-2-羟基戊基]-6,7-二甲氧基异色满-1-酮(2)、(+)-monocerin(3)、11-hydroxymonocerin(4)、5-丁基-6-(羟甲基)-4-甲氧基-2H-吡喃酮(5)和setophapyrone B(6)。结论 化合物1为新的聚酮类化合物,命名为突蠕孢酮。化合物3具有潜在的抗炎活性。Objective To study secondary metabolites of endophytic fungus Exserohilum rostratum hosted in Strychnos nux-vomica.Methods Separation and purification were carried out by various chromatographic separation methods such as silica gel column chromatography and HPLC. The structures of the isolated compounds were identified by HR-ESI-MS, NMR, IR, UV, and ECD. The compounds were tested for their anti-inflammatory activity by the Griess method. Results Six compounds, exserone A(1),(3R,4R)-4,8-dihydroxy-3-((R)-2-hydroxypentyl)-6,7-dimetho-xyisochroman-1-one(2),(+)-monocerin(3), 11-hydroxymonocerin(4), 5-butyl-6-(hydroxymethyl)-4-methoxy-2H-pyran-2-one(5) and setophapyrone B(6), were isolated from the fermentation products of endophytic fungus E. rostratum. Conclusion Compound 1 is a new polyketide and named exserone A. Compound 3 has potential anti-inflammatory activity.

关 键 词：马钱 内生真菌 次生代谢产物 抗炎 突蠕孢酮 (+)-monocerin 5-丁基-6-(羟甲基)-4-甲氧基-2H-吡喃酮 

分 类 号：R284.1[医药卫生—中药学]