茶皂素为乳化剂制备黄芩苷纳米乳及其促小鼠毛发生长的药效学研究  

Preparation of baicalin nanoemulsion with tea saponin as natural emulsifier and its pharmacodynamic study on promoting hair growth of C57BL/6 mice

作　　者：陈莉萍[1,2] 李小芳[2] 何春容[1] 洪雪梅 肖女 CHEN Li-ping;LI Xiao-fang;HE Chun-rong;HONG Xue-mei;XIAO Nyu(Xihua University,Chengdu 610039,China;Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Western Theater General Hospital,Chengdu 610083,China)

机构地区：[1]西华大学,四川成都610039 [2]成都中医药大学,四川成都610075 [3]西部战区总医院,四川成都610083

出　　处：《中草药》2023年第2期561-568,共8页Chinese Traditional and Herbal Drugs

基　　金：四川省科技计划项目(2020YFS0463);四川省中医药管理局科学技术研究专项项目(2021MS115);四川中医药大健康产业发展与乡村振兴研究中心资助项目(DJKZC202202)。

摘　　要：目的 以茶皂素为天然乳化剂制备黄芩苷纳米乳(baicalin nanoemulsion,Bai-NE),并探究其对C57BL/6小鼠毛发生长的促进作用。方法 采用超声乳化法制备Bai-NE,利用单因素实验法对Bai-NE的处方和制备工艺进行优化,并对最优处方和工艺制备的Bai-NE进行理化性质和稳定性考察。以C57BL/6小鼠为研究对象,评价Bai-NE对毛发生长的促进作用。结果 Bai-NE的最佳处方为茶皂素用量为0.5%,油相用量为5%,最佳制备工艺为超声功率180W,超声时间10min,超声次数1次。制得的Bai-NE为O/W型乳液,外观均一,平均粒径为(225.1±0.6)nm,多分散指数(polydispersity index,PDI)为0.082±0.031,载药量为1.10mg/g,pH值为7.25±0.20。稳定性结果显示,Bai-NE具有良好的离心稳定性和储存稳定性。Bai-NE能显著促进C57BL/6小鼠的毛发生长。结论 茶皂素可作为一种潜在的天然乳化剂用于Bai-NE的制备,制备的Bai-NE对C57BL/6小鼠的毛发生长有促进作用。Objective To prepare baicalin nanoemulsion(Bai-NE) using tea saponins(TS) as a natural emulsifier, and to explore the promoting effect of Bai-NE on the hair growth of C57BL/6 mice. Methods The Bai-NE was prepared by phacoemulsification. The optimal prescription and process parameters were screened out by single factor test, and the physicochemical property and stability of the Bai-NE prepared by the optimal prescription and process parameters were studied. C57BL/6 mice were used to evaluate the effect of the Bai-NE on hair growth. Results The best prescription of the Bai-NE was 0.5% of tea saponin and 5% of oil phase. The best preparation technology was ultrasonic power 180 W, ultrasonic time 10 min and ultrasonic frequency one time. The prepared Bai-NE is an O/W lotion with uniform appearance, mean particle size of(225.1 ± 0.6) nm, polydispersity index(PDI) of 0.082 ± 0.031, drug loading of 1.10 mg/g, and pH of 7.25 ± 0.20. The stability results showed that the Bai-NE had good centrifugal stability and storage stability. Bai-NE can significantly promote the hair growth of C57BL/6 mice. Conclusion Tea saponin can be used as a potential natural emulsifier for the preparation of Bai-NE, which can promote the hair growth of C57BL/6 mice.

关 键 词：茶皂素 黄芩苷 纳米乳 脂溢性脱发 药效学 超声乳化法 

分 类 号：R283.6[医药卫生—中药学]