基于GEO差异基因分析及网络药理学探究真人养脏汤治疗活动性溃疡性结肠炎的分子机制研究  

A Study on Mechanism of Zhenren Yangzang Decoction in the Treatment of Active Ulcerative Colitis Based on GEO Difference Analysis and Network Pharmacology

作　　者：尹世杰 王跃 程丽敏 YIN Shi-jie;WANG Yue;CHENG Li-min(The First Clinical Medical College,Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,Heilongjiang,China;Department of Anorectal Surgery,the First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,Heilongjiang,China)

机构地区：[1]黑龙江中医药大学第一临床医学院,黑龙江哈尔滨150000 [2]黑龙江中医药大学第一附属医院肛肠科,黑龙江哈尔滨150000

出　　处：《医学信息》2023年第3期70-76,共7页Journal of Medical Information

摘　　要：目的通过GEO差异基因分析及网络药理学探究真人养脏汤治疗活动性溃疡性结肠炎的分子机制。方法从GEO数据库检索溃疡性结肠炎,选择成年人活动期样本,并下载相关样本文件,再以R语言进行差异基因分析,获得相关靶点;从TCMSP数据库检索真人养脏汤各组成有效化合物成份及其对应的相关靶点,提取差异基因与相关药物靶点的交集,在STRING数据库中查询其蛋白互相作用网络(PPI),使用Cytoscape软件构建“药物-成分-靶点”调控网络并提取核心靶点,再利用R语言对其进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果筛得差异基因1097个,查得真人养脏汤中有效成分141个,对应靶点258个;从其中36个交集靶点中筛选出PPI核心靶点10个,关键靶点涉及白介素6(IL-6)、肿瘤坏死因子(TNF)、基质金属蛋白酶9(MMP9),基因显著富集于对脂多糖反应、受体配体活动、信号受体激活因子活性等分子功能。通路主要富集于肿瘤坏死因子(TNF)、白介素17(IL-17)、核转录因子κB(NF-κB)信号通路。结论真人养脏汤治疗UC具有多靶点、多通路等特点,尤其在炎性反应通路及免疫相关通路发挥重要作用。Objective To explore the molecular mechanism of Zhenren Yangzang Decoction in the treatment of active ulcerative colitis by GEO differential gene analysis and network pharmacology.Methods Ulcerative colitis was retrieved from the GEO database,and the active samples of adults were selected.The relevant sample files were downloaded,and the differential gene analysis was performed in R language to obtain the relevant targets.The effective compound components of Zhenren Yangzang decoction and their corresponding related targets were retrieved from the TCMSP database,and the intersection of differential genes and related drug targets was extracted.The protein interaction network(PPI)was queried in the STRING database.Cytoscape software was used to construct the“drug-component-target”regulatory network and extract the core targets,and then R language was used for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Results A total of 1097differential genes were screened,and 141 effective components and 258 corresponding targets were found in Zhenren Yangzang decoction.Ten PPI core targets were screened from 36 intersection targets.The key targets involved interleukin 6(IL-6),tumor necrosis factor(TNF),and matrix metalloproteinase 9(MMP9).The genes were significantly enriched in molecular functions such as lipopolysaccharide response,receptor ligand activity,and signal receptor activator activity.The pathways were mainly enriched in tumor necrosis factor(TNF),interleukin 17(IL-17),and nuclear transcription factorκB(NF-κB)signaling pathways.Conclusion Zhenren Yangzang decoction has the characteristics of multi-target and multi-pathway in the treatment of UC,especially in the inflammatory response pathway and immune-related pathway.

关 键 词：溃疡性结肠炎 GEO差异基因分析 网络药理 真人养脏汤 

分 类 号：R285.5[医药卫生—中药学] R574.62[医药卫生—中医学]