辣蓼黄酮乙酸乙酯部分抑制炎症因子产生及p38丝裂原活化蛋白激酶和细胞外调节蛋白激酶1/2蛋白磷酸化调节猪圆环病毒2型诱导的炎症反应  

Ethyl Acetate Fraction from Polygonum hydropiper L.Regulates Inflammatory Response Induced by Porcine Circovirus Type 2 by Reducing Production of Inflammatory Factors and Inhibiting Phosphorylation of p38 Mitogen-Activated Protein Kinase and Extracellular

作　　者：陈奇 韦玉衡 谢小东 赵怡 王秋华[1] 于美玲 韦英益[1] 胡庭俊[1] CHEN Qi;WEI Yuheng;XIE Xiaodong;ZHAO Yi;WANG Qiuhua;YU Meiling;WEI Yingyi;HU Tingjun(College of Animal Science and Technology,Guangxi University,Nanning 530005,China)

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《动物营养学报》2023年第2期1250-1260,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(32072907)。

摘　　要：本研究旨在探索辣蓼黄酮乙酸乙酯部分(FEA)抵御猪圆环病毒2型(PCV2)感染猪肺泡巨噬细胞(3D4/2细胞)诱导的炎症反应的分子机理。试验共设置7个组,分别为空白对照组、PCV2感染组、脂多糖阳性对照组、芦丁阳性对照组和FEA药物组(25、50和100μg/mL),每组4个重复。检测不同浓度FEA作用3D4/2细胞后的细胞活性;接种PCV2后,检测白细胞介素-6(IL-6)、干扰素-γ(IFN-γ)、白细胞介素-10(IL-10)含量及环氧合酶-1(COX-1)、环氧合酶-2(COX-2)活性;定量PCR检测COX-2、IL-6、IL-10、原癌基因(c-myc和c-fos)、氨基酸端激酶(c-jun)、p38丝裂原活化蛋白激酶(p38 MAPK)和细胞外调节蛋白激酶1/2(ERK1/2)的mRNA相对表达水平;Western-Blotting检测p38 MAPK、ERK1/2的蛋白相对表达水平。结果表明,25、50和100μg/mL的FEA对3D4/2细胞活力无显著影响(P>0.05)。与PCV2感染组相比,25、50和100μg/mL FEA药物组的IL-6、IL-10和IFN-γ含量显著或极显著降低(P<0.05或P<0.01),25和100μg/mL FEA药物组的COX-1和COX-2活性显著降低(P<0.05),25、50和100μg/mL FEA药物组的IL-6、IL-10、COX-2和c-fos的mRNA相对表达水平显著或极显著降低(P<0.05或P<0.01),25和100μg/mL FEA药物组的c-jun、c-myc、MAPK和ERK的mRNA相对表达水平显著或极显著降低(P<0.05或P<0.01),25、50和100μg/mL FEA药物组的p38 MAPK、ERK1/2的蛋白相对表达水平极显著降低(P<0.01)。由此可见,FEA通过减少炎症因子的产生并抑制p38 MAPK和ERK1/2蛋白磷酸化而调节PCV2诱导的3D4/2细胞的炎症反应。The objective of this study was to explore the molecular mechanism of ethyl acetate fraction from Polygonum hydropiper L.(FEA)resistance to the inflammatory response induced by porcine circovirus type 2(PCV2)infection in porcine alveolar macrophages(3D4/2 cells).The experiment was divided into seven groups,which were blank control group,PCV2 infection group,lipopolysaccharide positive control group,rutin positive control group and FEA drug groups(25,50 and 100μg/mL),each group contained 4 replicates.The cell viability of 3D4/2 cells after treated with different concentrations of FEA were detected;after inoculation of PCV2,the contents of interleukin-6(IL-6),interferon-γ(IFN-γ),interleukin-10(IL-10)and activities of cyclooxygenase-1(COX-1)and cyclooxygenase-2(COX-2)were detected;the mRNA relative expression levels of COX-2,IL-6,IL-10,proto-oncogene(c-myc and c-fos),amino acid end kinase(c-jun),p38 mitogen-activated protein kinase(p38 MAPK)and extracellular regulatory protein kinase 1/2(ERK1/2)were detected by quantitative PCR;the protein relative expression levels of p38 MAPK and ERK1/2 were detected by Western-Blotting.The results showed that 25,50 and 100μg/mL FEA had no effect on activity of 3D4/2 cells.Compared with PCV2 infection group,the contents of IL-6,IL-10 and IFN-γof 25,50 and 100μg/mL FEA drug groups were significantly decreased(P<0.05 or P<0.01),the activities of COX-1 and COX-2 of 25 and 100μg/mL FEA drug groups were significantly decreased(P<0.05),the mRNA relative expression levels of IL-6,IL-10,COX-2 and c-fos of 25,50 and 100μg/mL FEA drug groups were significantly decreased(P<0.05 or P<0.01),the mRNA relative expression levels of c-jun,c-myc,MAPK and ERK of 25 and 100μg/mL FEA drug groups were significantly decreased(P<0.05 or P<0.01),and the protein relative expression levels of p38 MAPK and ERK1/2 of 25,50 and 100μg/mL FEA drug groups were significantly decreased(P<0.01).It is shown that FEA regulates the inflammatory response of 3D4/2 cells induced by PCV2 by reducing the productio

关 键 词：炎症 猪圆环病毒2型 辣蓼黄酮 

分 类 号：S811.3[农业科学—畜牧学]