紫外线、高压静电和大麦黄矮病毒胁迫下荻草谷网蚜SOD基因表达  

Gene expression analysis of SOD gene under stresses of ultraviolet radiation,high-voltage electrostatic field and barley yellow dwarf virus in Indian grain aphid Sitobion miscanthi

作　　者：刘志伟 柳欣 董尧 罗晨[1] 胡祖庆[1] Liu Zhiwei;Liu Xin;Dong Yao;Luo Chen;Hu Zuqing(State Key Laboratory of Crop Stress Biology in Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi Province,China)

机构地区：[1]西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100

出　　处：《植物保护学报》2023年第1期91-100,共10页Journal of Plant Protection

基　　金：国家重点研发计划(2018YFD0200402);国家自然科学基金(31401735)。

摘　　要：为探索环境胁迫影响昆虫适合度的内在机制,通过cDNA末端快速扩增(rapid amplification of c DNA end,RACE)技术克隆得到荻草谷网蚜Sitobion miscanthi体内超氧化物歧化酶(superoxide dismutase,SOD)基因MnSOD和CuZnSOD的cDNA序列全长,对该序列进行分析,检测紫外线、高压静电和大麦黄矮病毒(barley yellow dwarf virus,BYDV)胁迫下荻草谷网蚜体内MnSOD和CuZnSOD基因表达量的变化。结果显示,荻草谷网蚜体内MnSOD和CuZnSOD基因的cDNA序列全长分别为1517 bp和954 bp(GenBank登录号分别为MT533627和MT533626),开放阅读框分别为669 bp和459 bp,分别编码222个和152个氨基酸,预测蛋白质相对分子量分别为24.99 kD和15.84 kD。荻草谷网蚜体内MnSOD和CuZnSOD蛋白均与半翅目蚜科昆虫同源蛋白氨基酸序列相似性高,亲缘关系最近。0.50 m W/cm^(2)(低强度)和0.70 m W/cm^(2)(高强度)紫外线胁迫下荻草谷网蚜体内MnSOD基因相对表达量显著上调,分别为对照的2.55倍和1.40倍,高压静电和BYDV胁迫下荻草谷网蚜体内MnSOD基因相对表达量显著下调,分别为对照的32%和14%;但低强度和高强度紫外线胁迫下荻草谷网蚜体内CuZnSOD基因相对表达量均较对照无显著变化,高压静电和BYDV胁迫下荻草谷网蚜体内CuZnSOD基因相对表达量均显著下调,分别为对照的51%和20%。表明SOD基因在荻草谷网蚜适应紫外线、高压静电和BYDV三种环境胁迫中发挥着重要作用。In order to explore the internal mechanism of environmental stress affecting insect fitness,rapid amplification of cDNA end(RACE)technology was used to obtain the full-length of cDNA sequences of superoxide dismutase(SOD)genes(MnSOD and CuZnSOD)in Indian grain aphid Sitobion miscanthi.Then,based on bioinformatics analysis,the expression levels of MnSOD and CuZnSOD genes under the stress of ultraviolet radiation(UV)treatment,high-voltage static electrostatic treatment,or plant virus infection by barley yellow dwarf virus(BYDV)were detected.Cloning results showed that the full lengths of MnSOD and CuZnSOD cDNAs in S.miscanthi were 1517 and 954 bases(GenBank accession nos.MT533627 and MT533626),with an open reading frame of 669 and 459 bases,encoding 222 and 152 amino acid residues,respectively.The molecular weights of the predicted proteins were 24.99 kD and 15.84 kD,respectively.MnSOD and CuZnSOD in S.miscanthi had higher amino acid sequence identity and the closest relationship with their homologues from Aphididae of Hemiptera.Compared with the control,the expression level of MnSOD in S.miscanthi was increased by 255%under high-level UV treatment and 140%under low-level UV treatment,but decreased by 68%under high-voltage static electrostatic treatment and by 86%under BYDV infection.CuZnSOD in S.miscanthi had a reduced expression by 49%under high-voltage static electrostatic treatment and by 80%under BYDV infection,and no difference was observed under the stress of UV treatment.Therefore,superoxide dismutase might play an important role in the adaptation of S.miscanthi to the above three adverse environmental stresses.

关 键 词：荻草谷网蚜 超氧化物歧化酶 MNSOD CUZNSOD 基因克隆 环境胁迫 基因表达 

分 类 号：S435.123[农业科学—农业昆虫与害虫防治]