辛酸乙酯对橘小实蝇产卵的驱避作用  

Repellent effects of ethyl caprylate on the oviposition of oriental fruit fly Bactrocera dorsalis

作　　者：李慧静 谢明学 程代凤 陆永跃 Li Huijing;Xie Mingxue;Cheng Daifeng;Lu Yongyue(College of Plant Protection,South China Agricultural University,Guangzhou 510642,Guangdong Province,China)

机构地区：[1]华南农业大学植物保护学院,广州510642

出　　处：《植物保护学报》2023年第1期153-160,共8页Journal of Plant Protection

基　　金：广东省自然科学基金(2019A1515012191)。

摘　　要：为进一步明确驱避橘小实蝇Bactrocera dorsalis产卵的挥发物成分,于室内测试橘小实蝇对被产卵不同时间的番石榴果实的产卵偏好,利用气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)联用技术、气相色谱-触角电位(gas chromatography-electroantennographic detection,GC-EAD)联用技术和生物测试等方法鉴定被产卵不用时间的番石榴果实中可驱避橘小实蝇产卵的挥发物。结果表明,被产卵48 h后的番石榴果实可显著驱避橘小实蝇产卵,橘小实蝇在未被产卵和被产卵番石榴果实中的产卵量分别为238.9粒和90.7粒。被产卵48 h后的番石榴果实中产生了辛酸乙酯,该物质可引起橘小实蝇雌成虫触角发生电生理反应。0.25、0.5、1和2μL/mL的辛酸乙酯均可驱避橘小实蝇雌成虫,驱避率分别为28%、48%、48%和28%。含有辛酸乙酯浓度分别为0.25、0.5、1和2μL/g的番石榴果泥可显著驱避橘小实蝇产卵,果泥中被产卵量分别为105.7、125.5、100.8和157.9粒,显著低于对照。番石榴果实表面分别涂抹浓度为50、100和200μL/mL辛酸乙酯后,在选择试验中,番石榴中橘小实蝇幼虫数量分别为164.5、27.5和4.8头;非选择性试验中,番石榴中橘小实蝇幼虫数量分别为187.6、95.2和30.6头,均显著低于对照。表明辛酸乙酯可显著驱避橘小实蝇雌成虫的产卵,具有开发成橘小实蝇驱避剂的潜力。To further clarify the volatile components repelling oviposition of oriental fruit fly Bactrocera dorsalis,the preference of B.dorsalis to guava was determined at different time points after oviposition;besides,the volatile components that repel B.dorsalis in egg-infested guava were identified with gas chromatography-mass spectrometry(GC-MS),gas chromatography-electroantennographic detection(GC-EAD)and bioassay.The results showed that the guava with oviposited eggs could significantly deter oviposition behavior of B.dorsalis at 48 h,and the number of eggs laid in non-infested and egginfested guavas was 238.9 and 90.7,respectively.Ethyl caprylate was detected in guava at 48 h after the initial egg deposition,which could elicit electrophysiological response from the female fly antenna.Bioassay results showed that ethyl caprylate at the concentrations of 0.25,0.5,1 and 2μL/mL could deter B.dorsalis and the repellence rates were 28%,48%,48%and 28%,respectively.Guava puree containing 0.25,0.5,1 and 2μL/g ethyl caprylate could significantly repel the oviposition of B.dorsalis,and the number of eggs in the treated puree was 105.7,125.5,100.8 and 157.9,respectively,which were significantly lower than the corresponding controls.After coating with ethyl caprylate at the concentrations of 50,100 and 200μL/mL,in choice test,the number of B.dorsalis larvae in guava fruits was 164.5,27.5 and 4.8,respectively;in no-choice test,the number of B.dorsalis larvae in guava fruits was 187.6,95.2 and 30.6,respectively,which were significantly lower than the corresponding controls.These results indicated that ethyl caprylate had a significant repellent effect on the oviposition of B.dorsalis.It had the potential for being developed as a repellent.

关 键 词：橘小实蝇 番石榴 挥发物 寄主选择 驱避 

分 类 号：TQ453.4[化学工程—农药化工]