僵蚕四草汤对咪喹莫特诱导银屑病样小鼠模型的治疗效果及机制研究  

Study on the therapeutic effect and mechanism of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model

作　　者：段利娜 任裕 景慧玲 王星星 王维英 DUAN Lina;REN Yu;JING Huiling;WANG Xingxing;WANG Weiying(The First Clinical Medical College,Shaanxi University of Chinese Medicine,Shaanxi Province,Xianyang712046,China;Department of Dermatology,Xi’an Hospital of Traditional Chinese Medicine,Shaanxi Province,Xi’an710021,China;Department of Nephrology,Xi’an Hospital of Traditional Chinese Medicine,Shaanxi Province,Xi’an710021,China)

机构地区：[1]陕西中医药大学第一临床医学院,陕西咸阳712046 [2]西安市中医医院皮肤科,陕西西安710021 [3]西安市中医医院肾病科,陕西西安710021

出　　处：《中国医药导报》2023年第6期28-32,共5页China Medical Herald

基　　金：陕西省西安市卫生健康委员会科研项目(2020ms08);陕西省自然科学基础研究计划项目(2020JM-695);陕西省中医药管理局市级中医医院科研能力提升项目(SZY-NLTL-2022-004);陕西中医药大学科学研究项目(2020FS02)。

摘　　要：目的探讨僵蚕四草汤对咪喹莫特诱导银屑病样小鼠模型的治疗效果并讨论其作用机制。方法将60只6~8周SPF级雄性BALB/c小鼠分为空白组,模型组,阳性药物对照组,僵蚕四草汤高、中、低浓度组,每组10只。除空白组外,其余5组小鼠均采用5%咪喹莫特乳膏62.5 mg涂抹背部制备银屑病样小鼠模型,同时僵蚕四草汤高、中、低浓度组分别给予20、10、5 mg/kg的僵蚕四草汤混悬液灌胃。阳性药物对照组给予阿维A 3.75 mg/kg的蒸馏水混悬液灌胃;空白组、模型组给予生理盐水灌胃,1次/d,连续7 d。比较各组小鼠皮损面积、组织病理、银屑病面积与严重程度指数(PASI)评分及血清白细胞介素(IL)-17A、IL-23、肿瘤坏死因子α(TNF-α)、生长转化因子β(TGF-β)、肿瘤坏死因子样细胞凋亡弱诱导剂(TWEAK)水平。结果模型组PASI评分高于空白组(P<0.05);僵蚕四草汤高、中、低浓度组PASI评分低于模型组(P<0.05);僵蚕四草汤组中浓度组PASI评分低于僵蚕四草汤组低浓度组,僵蚕四草汤组高浓度组PASI评分高于僵蚕四草汤组中浓度组(P<0.05)。造模给药第7天,空白组皮肤未表现出异常症状,其余各组都显示出不同程度的红斑、鳞屑、浸润;与模型组比较,僵蚕四草汤组的皮损程度均相对缓解;僵蚕四草汤中浓度组的皮损程度最低。空白组小鼠皮肤层次清楚明了,组织结构未见异常,表皮厚度正常;模型组小鼠皮损表现为类银屑病样改变,僵蚕四草汤组小鼠皮损可见表皮细胞角化程度减轻。模型组小鼠血清IL-17A、IL-23、TNF-α、TGF-β、TWEAK水平高于空白组(P<0.05);僵蚕四草汤组高、中、低浓度组血清IL-17A、IL-23、TNF-α、TGF-β、TWEAK水平低于模型组(P<0.05);僵蚕四草汤中浓度组小鼠血清IL-17A、IL-23、TNF-α、TGF-β、TWEAK水平低于僵蚕四草汤低浓度组,僵蚕四草汤高浓度组小鼠血清IL-17A、IL-23、TNF-α、TGF-β、TWEAK水平高于僵蚕四草�Objective To investigate the therapeutic effect of Jiangcan Sicao Decoction on imiquimod-induced psoriasis mice model,and to discuss its mechanism.Methods Sixty SPF male BALB/c mice of six to eight weeks were divided into blank group,model group,positive drug control group,Jiangcan Sicao Decoction high-concentration,medium-concentration,and low-concentration groups,with ten mice in each group.Except blank group,the other five groups were treated with 5%Imiquimod Cream 62.5 mg on the back to prepare psorias-like mouse model.Meanwhile,Jiangcan Sicao Decoction high-concentration,medium-concentration,and low-concentration groups were given 20,10,and 5 mg/kg suspension of Jiangcan Sicao Decoction by intragastric administration,respectively;positive drug control group was given 3.75 mg/kg of distilled water suspension of acitretin by gavage;blank group and model group were given normal saline intragastric administration,once per day for seven consecutive days.The skin area,histopathology,psoriasis area and severity index(PASI)score and serum levels of interleukin(IL)-17A,IL-23,tumor necrosis factorα(TNF-α),growth transforming factorβ(TGF-β),and tumor necrosis factor-like weak apoptosis inducer(TWEAK)were compared in each group.Results PASI score in model group was higher than that in blank group(P<0.05);PASI scores in Jiangcan Sicao Decoction high-concentration,medium-concentration,and low-concentration groups were lower than those in model group(P<0.05);PASI score of Jiangcan Sicao Decoction medium-concentration group was lower than that of Jiangcan Sicao Decoction low-concentration group,and PASI score of Jiangcan Sicao Decoction high-concentration was higher than that of Jiangcan Sicao Decoction medium-concentration group(P<0.05).On the 7th day of modeling administration,blank group showed no abnormal symptoms,and the other groups showed different degrees of erythema,scale,and infiltration;compared with model group,the skin lesions in Jiangcan Sicao Decoction group were relatively relieved,the degree of skin les

关 键 词：银屑病 僵蚕四草汤 小鼠 机制 

分 类 号：R259[医药卫生—中西医结合]