基于HPLC多指标成分、化学计量学和熵权优劣解距离法的苏木质量评价研究  

Quality evaluation of Sappan Lignum based on HPLC multi-index components,chemometrics combined with EW-TOPSIS method

作　　者：冯晓川[1] 王培[1] 徐延昭[2] 张蕊[1] FENG Xiao-chuan;WANG Pei;XU Yan-zhao;ZHANG Rui(Department of Chinese Pharmacy,Beijing Jishuitan Hospital,Beijing 100035,China;National Institutes for Food and Drug Control,Beijing 100050,China)

机构地区：[1]北京积水潭医院中药房,北京100035 [2]中国食品药品检定研究院,北京100050

出　　处：《现代药物与临床》2023年第1期77-84,共8页Drugs & Clinic

基　　金：北京市属医院科研培育计划中医项目(PZ2021005);北京市中医药管理局第二批中药骨干人才培养项目(京中医科字[2018]213号)。

摘　　要：目的 采用多指标成分、化学计量学联合熵权优劣解距离法综合评价苏木的质量。方法 采用HPLC法测定不同产地苏木中巴西苏木素、苏木精、原苏木素A、(±)原苏木素B、苏木酮A、鹰嘴豆芽素A、异甘草素和苏木查耳酮;对以上结果进行聚类分析、主成分分析、正交偏最小二乘法-判别分析,寻找影响苏木质量的主要潜在标志物;采用熵权优劣解距离法对不同产区苏木的质量优劣进行评价。结果 18批苏木聚为3类,不同产地苏木质量呈现一定的区域差异。苏木精、巴西苏木素、原苏木素A对苏木质量差异影响较大,是影响苏木质量的主要潜在标志物。广西、广东所产苏木质量最优,其次为四川、贵州和云南,福建地区产品质量较差。结论 本实验建立了苏木质量综合评价方法,操作便捷、可行性高、实用性强,为更加科学、系统地控制苏木质量提供技术支撑。Objective To comprehensively evaluate the quality of Sappan Lignum by multiple index, chemometrics, and EW-TOPSIS method. Methods HPLC method was used to simultaneously determine the contents of brazilin, hematoxylin, protosappanin A,(±)protosappanin B, sappanone A, biochanin A, isoliquiritigenin, and sappanchalcone in Sappan Lignum from different origins. The results were analyzed by clustering analysis(CA), principal component analysis(PCA), and orthogonal partial least squares-discriminant analysis(OPLS-DA) to look for the main potential markers affecting the quality of Sappan Lignum. Qualities of Sappan Lignum in different producing areas were evaluated by entropy TOPSIS(EW-TOPSIS) method. Results 18 Batches of Sappan Lignum could be clustered into 3 categories, and the quality of Sappan Lignum from different origins showed certain regional differences.Hematoxylin, brazilin, and protosappanin A had great influence on the quality difference of Sappan Lignum, and were the main potential markers affecting the quality of Sappan Lignum. The quality of Sappan Lignum from Guangxi and Guangdong Province was the best, followed by Sichuan, Guizhou and Yunnan Province, and that from Fujian Province was poor. Conclusion The established comprehensive evaluation method for the quality of Sappan Lignum is easy to operate, highly feasible, and practical, which can provide technical support for more scientific and systematic control of the quality of Sappan Lignum.

关 键 词：苏木 巴西苏木素 苏木精 原苏木素A 异甘草素 苏木查耳酮 聚类分析 主成分分析 正交偏最小二乘法-判别分析 熵权优劣解距离法 

分 类 号：R286.02[医药卫生—中药学]