百两金萜类代谢相关MYB转录因子挖掘及分析  

Analysis of MYB transcription factors related to terpenoids of Ardisia crispa

作　　者：李晓敏 赵春艳[2] 孙静贤 杨青松 高路 熊勇 LI Xiao-min;ZHAO Chun-yan;SUN Jing-xian;YANG Qing-song;GAO Lu;XIONG Yong(School of Ethnic Medicine,Yunnan Minzu University,Kunming 650500,China;All-China Federation of Supply and Marketing Cooperatives Kunming Institute of Edible Fungi,Kunming 650221,China)

机构地区：[1]云南民族大学民族医药学院,云南昆明650500 [2]中华全国供销合作总社昆明食用菌研究所,云南昆明650221

出　　处：《云南民族大学学报（自然科学版）》2022年第6期659-668,共10页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81760655);云南民族大学民族药资源化学国家民委-教育部重点实验室开放课题基金(MZY2104).

摘　　要：为探究百两金MYB转录因子参与萜类代谢生物途径,文中结合Mega 7.0、ORF、The MEME等软件,利用STRING、plantTFDB、KEGG、Mega 7.0、ORF、The MEME Suite等工具分析百两金MYB转录因子家族蛋白理化性质、系统进化、GO注释、蛋白功能等.结果获得了54条序列,MYB转录因子基因Cluster-20697.17470、Cluster-10301.0、Cluster-20709.0编码蛋白具有抑制肉桂酸4-羟化酶(C4H)的早期合成的作用,间接影响百两金的萜类产物代谢;推测Cluster-10535.0编码蛋白具有响应茉莉酸酯信号的作用.本研究在MYB转录因子家族中获得了参与萜类代谢途径基因Cluster-20709.0、Cluster-20697.17470、Cluster-10301.0和Cluster-10535.0,说明百两金转录因子参与了调节植物的生长以及次级代谢.研究参与萜类代谢生物途径的百两金MYB转录因子,为后续百两金MYB家族基因的挖掘和功能鉴定提供科学依据.In order to explore the biological pathways of the MYB transcription factors involved in terpenoid metabolism,in this study,combined with software such as Mega 7.0,ORF,the MEME,etc.,online databases such as STRING,plantTFDB,KEGG,etc.were used to analyze the physicochemical properties,system evolution,GO annotation,and protein function of Ardisia crispa MYB transcription factor family proteins.As a result,54 sequences were obtained.The MYB transcription factor genes Cluster-20697.17470,Cluster-10301.0,and Cluster-20709.0 encode proteins that inhibit the early synthesis of cinnamic acid 4-hydroxylase(C4H)and indirectly affect the terpenoidmetabolism of Ardisia crispa.It is speculated that the protein encoded by Cluster-10535.0 has the function of responding to jasmonate signal.In this study,the genes Cluster-20709.0,Cluster-20697.17470,Cluster-10301.0 and Cluster-10535.0 involved in the terpene metabolism pathway were obtained from the MYB transcription factor family,indicating that the Ardisia crispa transcription factor participates in the regulation of plant growth and secondary metabolism.The study of Ardisia crispa MYB transcription factors involved in the biological pathways of terpenoid metabolism will provide a scientific basis for the subsequent mining and functional identification of Ardisia crispa MYB family genes.

关 键 词：百两金 MYB转录因子 生物信息学 萜类代谢 

分 类 号：R285.5[医药卫生—中药学]