Klotho调控果蝇幼虫的运动行为  

Klotho regulates locomotor behavior of Drosophila larvae

作　　者：李港 胡君成 毛传樨 刘志华 金珊[1] LI Gang;HU Juncheng;MAO Chuanxi;LIU Zhihua;JIN Shan(School of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2023年第2期223-228,共6页Journal of Hubei University：Natural Science

基　　金：国家自然科学基金(81871121)资助。

摘　　要：klotho基因在人体内行使诸多重要功能,其在延长寿命、改善肾脏功能和缓解神经退行性疾病等方面都具有一定的作用,但其作用机制目前尚不明确.在果蝇中,人类klotho的同源基因为CG9701(klotho),目前与之相关的研究较少.本实验通过CRISPR/Cas9技术构建CG9701的无功能突变体klF13-3.klF13-3表现出运动速率降低,翻滚时间延长,但其运动轨迹正常,提示klotho主要在果蝇幼虫肌肉系统中发挥作用.我们的结果为后续klotho基因功能和调控机制的研究提供基础.Klotho is a multifunctional protein that has been shown to prolong lifespan,improve kidney function and alleviate neurodegenerative diseases,but the underlying mechanisms are unknown.Here we used Drosophila as a model to study its functions.The gene with the highest homology to human klotho in Drosophila was CG9701(klotho).We generated a klotho null mutant using the CRISPR/Cas9 system.We found that klotho promoted locomotor behavior of Drosophila larvae-klotho mutant larvae craw slower and took longer to roll.Our study provides a basis for subsequent studies on the function and regulation of the klotho.

关 键 词：KLOTHO CRISPR/Cas9 运动行为 

分 类 号：Q78[生物学—分子生物学]