限食影响模式生物家蚕Sir2等寿命相关基因的表达并延长寿命  

Dietary Restriction Affects Expression of Sir2 and Other Life-Span Related Genes and Prolongs Life-Span in Model Organism Silkworm Bombyx mori

作　　者：鲁月 王梅仙 胡超超 陈艳花 朱娟[1,2] 沈兴家[1,2] Lu Yue;Wang Meixian;Hu Chaochao;Chen Yanhua;Zhu Juan;Shen Xingjia(Jiangsu Key Laboratory of Sericulture Biology and Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212100,China;Key Laboratory of Sericulture Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212100,China)

机构地区：[1]江苏科技大学江苏省蚕桑生物学与生物技术重点实验室,江苏镇江212100 [2]中国农业科学院蚕业研究所农业农村部蚕桑遗传改良重点实验室,江苏镇江212100

出　　处：《蚕业科学》2022年第6期505-512,共8页ACTA SERICOLOGICA SINICA

基　　金：江苏省自然科学基金项目(BK20191226);江苏省研究生科研创新项目(KYCX21-3436)。

摘　　要：为探讨限食延长寿命的分子机制,以家蚕品种秋丰为研究对象,从2龄幼虫起限制采食(DR),每日1次给桑,自由采食16 h后强制停食8 h;以每日2次给桑的全天自由采食(AL)为对照。4-5龄期雌、雄分开饲养,选取3个与无脊椎动物寿命相关基因的家蚕同源基因BmSir2(Bombyx mori silencing information regulator 2)、BmAmpk(Bombyx mori adenosine activated protein kinase)和BmCox(Bombyx mori cytochrome C oxidase),利用qRT-PCR技术分析5龄3 d时的mRNA水平。进一步用RNAi技术敲减上述3个基因,调查其对成虫寿命的影响。结果显示,家蚕幼虫各组织中,3个寿命相关基因在DR组与AL组均呈现不同程度的表达差异;与雌性相比,限食后雄性的BmSir2、BmAmpk、BmCox在各组织中的表达上调更加明显;RNAi后3个基因的表达均显著下调,与对照组(dsGFP)相比成虫寿命显著缩短。结果表明限食促进寿命相关基因表达上调,且促进寿命延长。To investigate the molecular mechanism of dietary restriction in prolonging life-span,Qiufeng,a Bombyx mori bivoltine strain,was used as the research object.Dietary restriction(DR)groups of the 2 nd instar larvae were given mulberry leaves once a day,and then forced to fast for 8 h after being fed freely for 16 h.As a control,ad libitum feeding(AL)was given to mulberry twice a day with free feeding all day.The female and male larvae were reared separately from the 4 th to 5 th instars.The mRNA levels of BmSir2(Bombyx mori silencing information regulator 2),BmAmpk(Bombyx mori adenosine activated protein kinase)and BmCox(Bombyx mori cytochrome C oxidase),the silkworm homologs of three invertebrate life-span-related genes,were analyzed by qRT-PCR technology at the 5 th instar day-3 larvae.The above three genes were further knocked down by RNA interference(RNAi)technology to investigate their effects on adult lifespan.The results showed that the expression of three life-span related genes revealed differences in some way between the DR and the AL groups.BmSir2,BmAmpk,and BmCox genes were more significantly up-regulated in the tissues of male larvae in DR group compared with female.All three genes were significantly down-regulated after RNAi,and the adult life-spans were significantly shortened compared with the control group(dsGFP).These results indicate that dietary restriction up-regulates the expression of life-span related genes,and promotes adult life-span.

关 键 词：限食 家蚕 寿命 BmSir2 BmAmpk BmCox 

分 类 号：S881.2[农业科学—特种经济动物饲养]