不同饲料育条件下家蚕幼虫中肠细菌的多样性分析  

Diversity Analysis of Midgut Bacteria in Bombyx mori Larvae Reared with Different Diets

作　　者：荆文辉 占鹏飞 郝志华 钱秋杰 高云 徐豫松[1] 王华兵[1] Jing Wenhui;Zhan Pengfei;Hao Zhihua;Qian Qiujie;Gao Yun;Xu Yusong;Wang Huabing(College of Animal Sciences,Zhejiang University,Hangzhou 310058,China;Huzhou Academy of Agricultural Sciences,Huzhou Zhejiang 313000,China;Tongxiang Institute of Agricultural Sciences,Jiaxing Academy of Agricultural Sciences,Jiaxing Zhejiang 314500,China)

机构地区：[1]浙江大学动物科学学院,杭州310058 [2]湖州市农业科学研究院,浙江湖州313000 [3]嘉兴市农业科学研究院桐乡农业科学研究所,浙江嘉兴314500

出　　处：《蚕业科学》2022年第6期513-521,共9页ACTA SERICOLOGICA SINICA

基　　金：浙江省科学技术厅项目(LGN19C170002,2021C02072-6);浙江省自然科学基金项目(LR22C040001)。

摘　　要：为研究不同饲料饲育对家蚕幼虫中肠细菌多样性的影响,以家蚕品种秋丰×白玉为研究对象,分别以桑叶、NOSAN饲料和自制饲料饲育至5龄盛食期,取家蚕中肠样品进行16S rDNA高通量测序。结果发现,不同饲料育家蚕中肠的细菌菌群存在显著差异,NOSAN饲料育家蚕中肠的菌群丰度较高,而桑叶饲育组与自制饲料饲育组的菌群丰度相当。不同饲料育家蚕中肠菌群的功能基因在代谢途径上存在显著差异,且自制饲料饲育组与桑叶饲育组之间的差异明显大于NOSAN饲料饲育组与桑叶饲育组之间的差异。研究结果为开发优质家蚕人工饲料和探究肠道菌群对寄主的适应机制提供了参考。This study aims to investigate the effects of different diets on the bacterial diversity in midgut of Bombyx mori larvae.The silkworm variety Qiufeng×Baiyu was reared to the 5 th instar with mulberry leaf,NOSAN and homemade artificial diets,respectively,and the midgut samples were collected for 16 S rDNA high-throughput sequencing.The results showed that the midgut bacterial flora of silkworm larvae reared with different diets were significantly different.The flora abundance of larvae reared with NOSAN artificial diet was relatively higher,while the flora abundance of larvae reared with mulberry leaf was similar to that reared with homemade artificial diet.In addition,there were significant differences in the metabolic pathways of functional genes of midgut bacterial flora of silkworm reared with different diets,and the differences between larvae reared with homemade artificial diet and mulberry leaf was significantly higher than those between larvae reared with NOSAN artificial diet and mulberry leaf.This study lays a foundation for development of high quality artificial diet for silkworm larvae and exploring the adaptation mechanism of intestinal microflora to host.

关 键 词：家蚕 中肠 菌群丰度 16S rDNA 高通量测序 人工饲料 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q939[农业科学—畜牧兽医]