小麦Whirly基因家族的鉴定及表达分析  

Identification and Expression Analysis of Wheat(Triticum aestivum) Whirly Gene Family

作　　者：孙月 杨慧玉 朱文根 王小燕[1] 唐益苗[2] SUN Yue;YANG Hui-Yu;ZHU Wen-Gen;WANG Xiao-Yan;TANG Yi-Miao(College of Agriculture,Yangtze University,JingZhou 434000,China;Hybrid Wheat Research Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100000,China)

机构地区：[1]长江大学农学院,荆州434000 [2]北京市农林科学院杂交小麦研究所,北京100000

出　　处：《农业生物技术学报》2023年第2期223-231,共9页Journal of Agricultural Biotechnology

基　　金：北京市农林科学院协同创新中心资助项目(KJCX201907-2);北京市农林科学院科技创新能力建设专项(KJCX20200423)。

摘　　要：Whirly家族属于植物特有的转录因子,在多种植物生长发育、抗病和胁迫响应等方面发挥着重要作用。为探究小麦(Triticum aestivum)Whirly家族成员数量及潜在功能,本研究借助生物信息学方法,对鉴定到的6个TaWHYs成员(分别命名为TaWHY1-A,TaWHY1-A-1,TaWHY1-D,TaWHY2-A,TaWHY2-B,TaWHY2-D)进行蛋白理化性质、基因结构、蛋白三维结构及系统进化分析;使用qPCR对TaWHYs成员在干旱、高温、低温胁迫下的表达模式进行分析。结果表明,TaWHYs蛋白不稳定指数为30~60,亲水性平均系数小于0,蛋白性质稳定,属于亲水蛋白。亚细胞定位预测显示,TaWHYs蛋白在细胞核、细胞质、线粒体、叶绿体中均有分布。TaWHYs蛋白二级结构以α螺旋和无规则卷曲为主,存在2种三维结构模型。转录组数据分析表明,TaWHY基因表现出不同的组织表达模式,并受到低温、高温、干旱等不同非生物胁迫的调控。qPCR结果表明,TaWHY1-A基因在低温胁迫条件下相对表达量显著上调(P<0.05),能够响应低温胁迫。本研究结果为更深层次的TaWHY功能验证和作用机理分析提供参考。Whirly family is a kind of plant-specific transcription factors, which have extensive and complex biological functions in both the nucleus and organelles. Because of the rotating appearance of its quaternary structure, it is called Whirly protein, and the corresponding gene is named Whirly gene. This family plays an important role in plant growth and stress response. In order to explore the function of wheat(Triticum aestivum) Whirly family, the basic characteristics of TaWHY gene family members were analyzed by bioinformatics method, and their responses to various stresses were verified by qPCR. The results showed that there were 6 members in TaWHY family, named TaWHY1-A, TaWHY1-A-1, TaWHY1-D, TaWHY2-A, TaWHY2-B and TaWHY2-D, respectively, which were distributed on 6 chromosomes of wheat, and all the member sequences contained introns. The results showed that Ta WHYs protein instability index was 30~60, and the average coefficient of hydrophilicity was less than 0, which indicated that wheat Whirly protein was stabled in nature and belongs to hydrophilic protein. TaWHY protein was distributed in mitochondria, chloroplast,nucleus and cytoplasm. The secondary structure of TaWHY protein was mainly alpha helix and random curl,and there were 2 three-dimensional structure models. Transcriptional data analysis showed that TaWHY gene showed different tissue expression patterns and was regulated by different abiotic stresses. The results of qPCR showed that the relative expression of TaWHY1-A gene was significantly up-regulated under low temperature stress conditions(P<0.05), which could respond to low temperature stress. The results of this study can provide reference for further functional verification and mechanism analysis of TaWHY.

关 键 词：小麦 Whirly基因家族 生物信息学分析 非生物胁迫 表达分析 

分 类 号：S330[农业科学—作物遗传育种]