刺葡萄MYB基因家族鉴定及其在不同光质下的表达模式分析  

Identification of MYB Gene Family and Its Expression Pattern Analysis Under Different Light Quality in Spine Grape(Vitis davidii)

作　　者：阙秋霞 赖恭梯 潘若 张静 贺丽媛 赖谱富[2] 王琦[2] 高慧颖[2] 陈桂信[1] 赖呈纯[2] QUE Qiu-Xia;LAI Gong-Ti;PAN Ruo;ZHANG Jing;HE Li-Yuan;LAI Pu-Fu;WANG Qi;GAO Hui-Ying;CHEN Gui-Xin;LAI Cheng-Chun(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Agricultural Engineering Technology,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China)

机构地区：[1]福建农林大学园艺学院,福州350002 [2]福建省农业科学院农业工程技术研究所,福州350003

出　　处：《农业生物技术学报》2023年第2期298-310,共13页Journal of Agricultural Biotechnology

基　　金：福建省公益类科研院所专项(2021R10320013);福建省财政专项-科技创新团队项目(CXTD2021018-2);福建省农业科学院对外项目(DWHZ2021-01);福建省农业科学院自由探索科技创新项目(ZYTS2021008)。

摘　　要：MYB转录因子是植物生长发育和次生代谢中重要的调控因子,对花青素的生物合成有重要调控作用。本研究从刺葡萄(Vitis davidii)愈伤组织转录组数据鉴定并克隆出9个VdMYB家族基因,并利用生物信息学方法分析和预测其结构和功能,同时分析其在不同光质诱导下的表达模式。基因序列分析结果显示大部分MYB基因包括3个外显子和2个内含子;蛋白质理化性质分析表明,VdMYBs氨基酸残基数目为216~335个,相对分子量大小为24.09~36.17 kD,等电点为5.42~9.58;进化树分析表明,9个VdMYBs被划分为6个组群,预测分别定位于细胞质、线粒体和细胞核中;启动子顺式作用元件预测发现,9个VdMYB启动子序列中均有大量光响应等多种生物/非生物响应元件。qRT-PCR分析表明,光质显著影响VdMYBs的表达,不同光质作用下,VdMYBs基因响应模式与结构特征相对应;VdMYB31/VdMYBB1和VdMYB4A分别作为正负调控因子参与花青素生物合成。本研究为进一步解析MYB基因通过响应光信号参与调控刺葡萄愈伤组织花青素生物合成的分子机制提供理论参考。MYB transcription factor is an important regulatory factor in plant growth and secondary metabolism, and plays key roles in anthocyanin biosynthesis. In this research, 9 VdMYB genes were identified and cloned from Vitis davidii callus based on previous transcriptome data. The MYB gene structure and function were analyzed and predicated by bioinformatic softwares, and their expression patterns were analyzed under different light quality. The results showed that most MYB genes included 3 exons and 2 introns;the number of amino acid residues of VdMYBs were 216~335, the relative molecular mass were 24.09~36.17 kD,and the isoelectric point were 5.42~9.58. The phylogenetic tree analysis indicated that the 9 VdMYB proteins were divided into 6 groups, and were predicted to be located in cytoplasm, mitochondria and nucleus,respectively. Analysis of promoter cis-acting elements showed that a large number of light-response and bio/abio-response elements were predicted in the 9 VdMYB promoters. The qRT-PCR analysis showed that light quality significantly affected the expression of VdMYBs, their expression pattern corresponded to the structural characteristics under different light quality. Among 9 VdMYB genes, VdMYB31/VdMYBB1 and VdMYB4A might play as positive and negative regulators involved in anthocyanin biosynthesis. This study provides theoretical reference for further elucidating the light-response molecular mechanism of MYB family genes involved in the regulatory pathways of anthocyanins in Vitis davidii.

关 键 词：刺葡萄 愈伤组织 MYB转录因子 光质 表达分析 

分 类 号：S663.1[农业科学—果树学] Q786[农业科学—园艺学]