INHβB、FSHR和LHR在牦牛有腔卵泡中的表达与分布  

Expression and Distribution of INHβB, FSHR and LHR in Yak(Bos grunniens) Antral Follicles

作　　者：王军乾 赵凌 潘阳阳[1] 王萌[1] 黄振华[1] 杨贻 崔燕[1] 余四九[1,2] 王立斌 WANG Jun-Qian;ZHAO Ling;PAN Yang-Yang;WANG Meng;HUANG Zhen-Hua;YANG Yi;CUI Yan;YU Si-Jiu;WANG Li-Bin(College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;Gansu Province Livestock Embryo Engineering Research Center,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃省牛羊胚胎工程技术研究中心,兰州730070

出　　处：《农业生物技术学报》2023年第2期334-340,共7页Journal of Agricultural Biotechnology

基　　金：甘肃省农牧厅农业生物技术研究项目(GNSW-2015-19);甘肃省教育厅产业支撑引导项目(2019C-03);甘肃农业大学盛彤笙基金(GSAU-STS-2018-23)。

摘　　要：抑制素(inhibin, INH)、促卵泡素(follicle-stimulating hormone, FSH)和促黄体素(luteinizing hormone, LH)对于卵泡发育具有重要影响。本研究通过qPCR和Western blot技术分别检测INHβB、FSH受体(FSH receptor, FSHR)和LH受体(LH recepter, LHR)基因及蛋白在健康牦牛(Bos grunniens)不同直径有腔卵泡中的表达,同时采用免疫组织化学(immunohistochemistry, IHC)技术检测三者在牦牛有腔卵泡中的分布。结果显示,INHβB基因和蛋白表达量随着卵泡直径的增大呈先上升后下降的趋势,在大卵泡(直径5~8 mm)中表达量最高(P<0.05);FSHR在大卵泡中的基因和蛋白表达量显著高于其他直径的卵泡(P<0.05),在其他不同直径卵泡中的表达差异不显著;LHR基因和蛋白表达量随着卵泡直径的增大而不断上升,在极大卵泡(直径>8 mm)中表达量最高(P<0.05)。IHC结果显示,INHβB、FSHR和LHR主要分布于卵泡颗粒细胞、卵丘细胞和卵泡膜。上述结果提示,INHβB可能对牦牛卵泡发生、闭锁或排卵有重要影响,FSHR和LHR可能增强FSH和LH对卵泡生长发育的作用。本研究为提高牦牛繁殖效率提供参考依据。Inhibin(INH), follicle-stimulating hormone(FSH) and luteinizing hormone(LH) have important effects on the development of follicles. This study aimed to detect the expression of INHβB, FSH receptor(FSHR) and LH receptor(LHR) genes and proteins in yak(Bos grunniens) antral follicles with different diameters by qPCR and Western blot, and immunohistochemistry(IHC) was used to detect their distribution in yak antral follicles. The results showed that INHβB gene and protein expression in antral follicles increased first and then decreased with the increase of follicle diameter, and the highest expression level was found in large follicles(5~8 mm in diameter)(P<0.05). The FSHR gene and protein expression in large follicles(5~8mm in diameter) were significantly higher than that in other follicles(P<0.05), while the expression of FSHR in other follicles with different diameters were not significantly different. LHR gene and protein expression increased with the increase of follicles diameter, and the highest expression level was found in the extra large follicles(diameter>8 mm)(P<0.05). IHC results showed that INHβB, FSHR and LHR mainly distributed in granulosa cells, cumulus cells and follicle membrane. The above results indicates that INHβB might has an important effect on follicle atresia or ovulation in yaks, and FSHR and LHR might enhance the effect of FSH and LH on follicle growth and development. This study provides a reference for improving the reproductive efficiency of yaks.

关 键 词：牦牛 有腔卵泡 抑制素βB(INHβB) 促卵泡素受体(FSHR) 促黄体素受体(LHR) 

分 类 号：S823.85[农业科学—畜牧学]