湖羊GLP2R基因多态性及其与内脏重量的关联分析  

Polymorphism of GLP2R Gene and Its Association Analysis with Visceral Weight in Hu Sheep(Ovis aries)

作　　者：黄永亮 王维民[1] 汪晓娟[1] 王国秀[1] 马宗武 崔盼盼 张德印 李冲[1] HUANG Yong-Liang;WANG Wei-Min;WANG Xiao-Juan;WANG Guo-Xiu;MA Zong-Wu;CUI Pan-Pan;ZHANG De-Yin;LI Chong(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《农业生物技术学报》2023年第2期341-349,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31660670);国家畜禽良种联合攻关计划(19200140);国家肉羊产业技术体系(CARS-38);甘肃省自然基金(20JR10RA538)。

摘　　要：胰高血糖素样肽-2受体(glucagon-like peptide-2 receptor, GLP2R)是调控机体能量平衡的关键分子,可能对绵羊(Ovis aries)器官发育和生长性能具有重要影响。本研究旨在研究湖羊GLP2R基因单核苷酸多态性与屠宰性能以及心脏、肝脏、脾脏、肺脏和肾脏等内脏器官重量的关联性。首先利用qPCR分析GLP2R基因在不同组织中的表达谱,进一步利用竞争性等位基因特异性PCR(kompetitive allele specific PCR, KASP)分型技术对1 351只具有完整系谱的湖羊公羔GLP2R基因位点多态性进行检测,并与羔羊的屠宰性能和内脏器官重量进行关联分析。结果表明:GLP2R基因在湖羊多种组织中广泛表达,且在肾脏和瘤胃组织中的表达量显著高于其他组织(P<0.05)。在GLP2R基因外显子g.34409249 G>A位点存在3种基因型GG、GA和AA,表现为中度多态0.25A的突变位点可作为心脏、肺脏和肾脏器官发育的候选分子标记位点,为湖羊的选育工作提供了理论支持。Glucagon-like peptide-2 receptor(GLP2R) is one of the key mediators of regulating body energy balance, which may have important effects on organ development and growth performance of sheep(Ovis aries). This study was conducted to investigate the association between GLP2R single nucleotide polymorphism and slaughter performance and weight of internal organs such as heart, liver, spleen, lung and kidney in Hu sheep. Firstly, the expression of GLP2R gene in different tissues was analyzed by qPCR.Furthermore, the polymorphism of GLP2R gene locus was detected by kompetitive allele specific PCR(KASP) typing technique in 1 351 male Hu lamb with complete pedigree, and the correlation analysis was conducted with the slaughter traits and weight of internal organs of lambs. The results showed that GLP2R gene was widely expressed in various tissues of Hu sheep, and the relative expression level was significantly higher in kidney and rumen tissues(P<0.05). There were 3 genotypes GG, GA and AA at g.34409249 G>A locus of GLP2R gene exon, which showed moderate polymorphism(0.25A) can be used as a candidate molecular marker site for heart, lung and kidney organ development, which provides a theoretical basis for the breeding of Hu sheep.

关 键 词：湖羊 胰高血糖素样肽-2受体(GLP2R)基因 多态性 内脏重量 关联分析 

分 类 号：S813.3[农业科学—畜牧学]