不同丛枝菌根真菌组合对滇重楼根系生理特性的影响  

Effects of different Arbuscular mycorrhiza fungi combinations on root physiological characteristics of Paris polyphylla var.yunnanensis

作　　者：许凌峰 李卓蔚 郭冬琴 丁博 赵晶晶 周浓 XU Lingfeng;LI Zhuowei;GUO Dongqin;DING Bo;ZHAO Jingjing;ZHOU Nong(Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area′s Medicinal Herbs,College of Food and Biological Engineering,Chongqing Three Gorges University,Chongqing,404120 P.R.China)

机构地区：[1]重庆三峡学院生物与食品工程学院、三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120

出　　处：《华西药学杂志》2023年第1期65-69,共5页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81260622);重庆市自然科学基金项目(cstc2018jcyjAX0267);重庆市教委科学技术研究项目(KJQN202101239)。

摘　　要：目的 研究不同丛枝菌根真菌(AMF)组合对滇重楼根系生理特性的作用。方法 采用室内盆栽法,观察接种9种不同AMF组合对滇重楼菌根侵染强度、根系活力、丙二醛(MDA)、可溶性糖(SS)和可溶性蛋白(SP)含量以及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的影响。结果 与空白对照(不接种AMF)组比较,滇重楼苗期接种S6(球状巨孢囊霉、巨大巨孢囊霉、美丽盾巨孢囊霉、透明盾巨孢囊霉、近明球囊霉、沙荒球囊霉)和S7(球状巨孢囊霉、透明盾巨孢囊霉、玫瑰红巨孢囊霉、根内球囊霉、亮色盾巨孢囊霉、明球囊霉)组合菌剂可显著增加菌根侵染强度和根系活力,促进根系内SS和SP含量的增加,提高CAT、SOD、POD的活性,降低MDA的含量。结论 滇重楼苗期接种S6、S7组合菌剂可以有效地维持根系细胞膜系统的稳定性,延缓根系的衰老。OBJECTIVE To study the effect of different Arbuscular mycorrhizal fungi(AMF) combinations on the root physiological characteristics in Paris polyphylla var.yunnanensis.METHODS Indoor potted method was used, 9 groups of different AMF combinations were inoculated as treatment, and no vaccination was used as a blank control.The effect of different AMF combinations on mycorrhizal infection strength, root viability, malondialdehyde(MDA),soluble sugar(SS) and soluble protein(SP) content and activities of catalase(CAT),superoxide dismutase(SOD) and peroxidase(POD) were observed by indoor potting method.RESULTS Compared with the blank control, the mycorrhiza intensity and root vigor, SS and SP contents, CAT,SOD and POD activities increased, and MDA content declined by inoculation of S6(Gigaspora margarita,G.gigantea,Scutellospra calospora, S.ellucida,Septoglomus deserticola and Claroideoglomus claroideum) and S7(G.margarita,G.rosea,S.pellucida,Racocetra fulgida,Rhizophagus intraradices and R.claruus) at the seedling stage of P.polyphylla var.yunnanensis.CONCLUSION Inoculation of S6 and S7 combination at the seedling stage of P.polyphylla var.yunnanensis can effectively maintain the stability of root cell membrane system and delay the senescence of root system.

关 键 词：滇重楼 丛枝菌根真菌 根系 根系活力 菌根侵染强度 丙二醛 抗氧化酶 渗透调节物质 

分 类 号：R28[医药卫生—中药学] R917[医药卫生—中医学]