滋阴补脾法对人结肠癌裸鼠肿瘤标志物的影响  

Effect of Nourishing Yin and Replenishing Spleen Therapy on Tumor Markers in Nude Mice Bearing Human Colon Cancer

作　　者：李静 武如通 朱汉平[3] LI Jing;WU Ru-Tong;ZHU Han-Ping(Dept.of Spleen and Stomach Diseases,Guangdong Second Hospital of Traditional Chinese Medicine(Guangdong Academy of Engineering and Technology of Traditional Chinese Medicine),Guangzhou 510095 Guangdong,China;Dept.of Oncology,Guangdong Second Hospital of Traditional Chinese Medicine(Guangdong Academy of Engineering and Technology of Traditional Chinese Medicine),Guangzhou 510095 Guangdong,China;Dept.of Traditional Chinese Medicine,The First Affiliated Hospital of Guangzhou Medical University,Guangzhou 510120 Guangdong,Chinal)

机构地区：[1]广东省第二中医院(广东省中医药工程技术研究院)脾胃病科,广东广州510095 [2]广东省第二中医院(广东省中医药工程技术研究院)肿瘤病科,广东广州1510095 [3]广州医科大学附属第一医院中医科,广东广州1510120

出　　处：《广州中医药大学学报》2023年第1期177-182,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局科研课题(编号:20191033)。

摘　　要：【目的】探讨滋阴补脾法对人结肠癌裸鼠的治疗作用及机制。【方法】将32只裸鼠接种人结肠癌细胞COLO-320构建结肠癌移植瘤模型,随机分为4组,空白组(生理盐水)、化疗组(腹腔注射氟尿嘧啶)、中药组(灌胃滋阴补脾中药复方)、联合组(腹腔注射氟尿嘧啶+灌胃滋阴补脾中药复方),连续给药7 d。开始给药至给药后第7天,观察裸鼠肿瘤生长情况,采用Western Blot法检测裸鼠治疗前后肿瘤组织CD133、CD166、p53蛋白表达水平。【结果】(1)从开始治疗至治疗后第7天,空白组结肠癌裸鼠瘤体体积逐渐增长,化疗组、中药组以及联合组结肠癌裸鼠瘤体体积逐渐下降,与空白组同期比较,差异均有统计学意义(P<0.05)。(2)治疗后第7天,与空白组比较,化疗组、中药组以及联合组结肠癌裸鼠瘤体最大径、最小径及瘤体质量明显降低,抑瘤率增加,且联合组作用效果优于化疗组、中药组(均P<0.05)。(3)治疗前,各组结肠癌裸鼠肿瘤组织CD133、CD166以及p53蛋白表达水平均呈高表达,差异无统计学意义(P>0.05);治疗后,化疗组、中药组以及联合组结肠癌裸鼠肿瘤组织CD133、CD166以及p53蛋白表达水平均较治疗前降低(P<0.05),且联合组降低作用明显优于化疗组、中药组(P<0.05)。【结论】滋阴补脾法可抑制人结肠癌裸鼠肿瘤生长,降低结肠癌肿瘤标志物表达,有助于结肠癌化疗药物治疗。Objective To explore the therapeutic effect and mechanism of nourishing yin and replenishing spleen therapy on tumor markers in nude mice bearing human colon cancer.Methods Thirty-two nude mice were inoculated with human colon cancer cells COLO-320 to construct a colon cancer transplantation tumor model,and randomly divided into 4 groups:blank group(normal saline),chemotherapy group(intraperitoneal injection of fluorouracil),Chinese medicine group(intragastric administration of Chinese medicine compound for nourishing yin and replenishing spleen),and combination group(intraperitoneal injection of fluorouracil plus intragastric administration of Chinese medicine compound for nourishing yin and replenishing spleen).The treatment was administered continuously for 7 days.The tumor growth of the nude mice was observed from the beginning of administration to the 7th day after administration.The protein expression levels of CD133,CD166 and p53 in the tumor tissue of nude mice before and after treatment were measured by Western Blot.Results(1)From the beginning of treatment to the 7th day after treatment,the tumor volume of nude mice bearing colon cancer in the blank group was gradually increased,while the tumor volume of nude mice bearing colon cancer in the chemotherapy group,Chinese medicine group and the combination group were gradually decreased,the difference being statistically significant compared with the blank group at the same period(P<0.05).(2)On the7th day after treatment,compared with the blank group,the maximum diameter and the smallest diameter of tumor body and the tumor mass of nude mice bearing colon cancer in the chemotherapy group,the Chinese medicine group and the combination group were significantly reduced,and the tumor inhibition rate was increased,and the effect of the combination group was superior to that of the chemotherapy group and the Chinese medicine group(all P<0.05).(3)Before treatment,the protein expression levels of CD133,CD166 and p53 in the tumor tissue of nude mice bearing colon ca

关 键 词：滋阴补脾法 结肠癌 CD133 CD166 p53 裸鼠 

分 类 号：R285.5[医药卫生—中药学]