苗药搜山虎对糖尿病痛性神经病变大鼠镇痛因子及相关痛阈影响研究  

Effect of Miao Medicine Crocosmia Crocosmiflora on Analgesic Factors and Related Pain Threshold in Diabetic Painful Neuropathy Rats

作　　者：刘丹 倪洪岗 邓闵心 黄晓芳 刘信 Liu Dan;Ni Honggang;Deng Minxin;Huang Xiaofang;Liu Xin(Graduate School,Guizhou University of Traditional Chinese Medicine,Guiyang 550002,China;The Second Hospital Affiliated to Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China)

机构地区：[1]贵州中医药大学研究生院,贵州贵阳550002 [2]贵州中医药大学第二附属医院,贵州贵阳550003

出　　处：《亚太传统医药》2023年第1期17-22,共6页Asia-Pacific Traditional Medicine

摘　　要：目的:通过观察不同剂量苗药搜山虎对糖尿病痛性神经病变(PDN)大鼠镇痛因子及相关机械痛阈值、热痛阈值的影响,探讨苗药搜山虎对PDN大鼠的镇痛机制,为其治疗提供研究依据。方法:随机将81只SPF级雄性Wistar大鼠分为正常对照组、模型组、硫辛酸组、搜山虎低、中、高剂量组。采用链脲佐菌素(STZ)溶液腹腔注射结合饮食干预的方法复制PDN大鼠模型。造模成功后,硫辛酸组予硫辛酸62.5 mg/kg灌胃,搜山虎低、中、高剂量组分别予搜山虎0.31、0.62、1.24 g/kg灌胃,正常对照组和模型组以生理盐水灌胃,每日给药1次,每组给药时间为4周。治疗结束后采用ELISA法检测大鼠血清内IL-2、IL-4、IL-10及β-EP水平,测定其机械痛阈值、热痛阈值。结果:与正常对照组比较,模型组IL-2、IL-4、IL-10、β-EP含量均明显减少(P<0.01),机械痛阈值和热痛阈值均明显降低(P<0.01);与模型组比较,搜山虎组、硫辛酸组IL-2、IL-4、IL-10、β-EP含量均明显升高(P<0.01),机械痛阈值和热痛阈值均明显升高(P<0.01)。结论:苗药搜山虎可以上调PDN大鼠血清中IL-2、IL-4、IL-10及β-EP的水平,并提高PDN大鼠机械痛阈值和热痛阈值,从而缓解疼痛症状。Objective:By observing the effects of different doses of Miao Medicine Crocosmia Crocosmiflora on analgesic factors and related the mechanical and thermal pain thresholds in Painful Diabetic Neuropathy(PDN) rats, the analgesic mechanism of Miao Medicine Crocosmia Crocosmiflora on PDN rats was explored, and the research basis was provided for the treatment of PDN by Miao Medicine Soushanhu.Methods:81 SPF male Wistar rats were randomly divided into normal control group, model group, lipoic acid group, low, medium and high dose groups of Crocosmia Crocosmiflora.PDN rat models were established by injecting Streptozotocin(STZ) solution intraperitoneally and intervening in the diet.After the successful modeling, lipoic acid group was given lipoic acid in the dose of 62.5 mg/kg by gavage;low, medium and high dose groups of Crocosmia Crocosmiflora were given Crocosmia Crocosmiflora in the doses of 0.31,0.62 and 1.24 g/kg by gavage respectively;normal control group and model group were given normal saline by gavage, once a day, for 4 weeks in each group.After the treatment, the serum levels of IL-2,IL-4,IL-10 and β-EP were detected by ELISA,and the mechanical and thermal pain thresholds were tested.Results:Compared with normal control group, the contents of IL-2,IL-4,IL-10 and β-EP in model group significantly decreased(P<0.01),and the mechanical and thermal pain thresholds significantly decreased(P<0.01).Compared with model group, the contents of IL-2,IL-4,IL-10 and β-EP in lipoic acid group and Crocosmia Crocosmiflora group significantly increased(P<0.01),and the mechanical and thermal pain thresholds in lipoic acid group and Crocosmia Crocosmiflora group significantly increased(P<0.01).Conclusion:Miao Medicine Crocosmia Crocosmiflora can significantly up-regulate the levels of IL-2,IL-4,IL-10 and β-EP in serum of PDN rats, and improve the mechanical and thermal pain thresholds of PDN rats, so as to relieve pain symptoms.

关 键 词：搜山虎 苗药 糖尿病痛性神经病变 镇痛因子 机械痛阈值 热痛阈值 

分 类 号：R29[医药卫生—民族医学] R285.5[医药卫生—中医学]