不同寄主饲养草地贪夜蛾的遗传分析  

Genetic Analysis of Spodoptera frugiperda Reared on Different Hosts

作　　者：贾文珍 罗莲 马春虎 姜秀萍 巴红国 梁越洋[1] 李平[1] 李双成[1] 王世全[1] JIA Wenzhen;LUO Lian;MA Chunhu;JIANG Xiuping;BA Hongguo;LIANG Yueyang;LI Ping;LI Shuangcheng;WANG Shiquan(Rice Research Institute of Sichuan Agricultural Univercity,Chengdu 611130,China)

机构地区：[1]四川农业大学水稻研究所,成都611130

出　　处：《四川农业大学学报》2023年第1期61-67,共7页Journal of Sichuan Agricultural University

基　　金：转基因生物新品种培育重大专项(2016ZX08001-001)。

摘　　要：【目的】鉴定入侵四川省草地贪夜蛾的亚型,使用不同寄主饲养,初步探索其遗传特征。【方法】利用线粒体细胞色素C氧化酶亚基Ⅰ基因(cytochrome coxidase subunitⅠ,COⅠ),以及位于Z染色体上的磷酸甘油醛异构酶基因(triose-phosphate isomerase,Tpi)2个分子标记对采集自中国四川省的草地贪夜蛾进行遗传分析。【结果】基于COⅠ基因的鉴定结果显示50份采集自四川的草地贪夜蛾样品均为“玉米型”;后续使用人工饲料、玉米和水稻幼苗3种寄主植物分别连续喂养至第三代发现,人工饲料和玉米幼苗喂养的草地贪夜蛾均为“玉米型”,取自水稻幼苗的草地贪夜蛾样品则部分出现“水稻型”;而基于Tpi基因的分析表明所有样品均为“玉米型”。第6代样品鉴定结果显示,水稻幼苗的草地贪夜蛾样品中基于COⅠ基因鉴定出的“水稻型”增多,并且部分样品的Tpi基因鉴定为“水稻型”,而取自人工饲料和玉米幼苗的草地贪夜蛾样品均为“玉米型”。【结论】采集的入侵四川省的草地贪夜蛾可能并不是单一的亚型,部分存在“杂合型”。草地贪夜蛾的主要寄主植物玉米、水稻等在四川省均有种植,厘清该虫的遗传特征,可为农业部门制定针对性防控对策提供科学依据。【Objective】This study aimed to identify the subtypes of Spodoptera frugiperda invasive in Sichuan province,feed them with different hosts,and explore their genetic characteristics preliminarily.【Method】In this study,two molecular markers,cytochrome coxidase subunitⅠ(COⅠ)and triosephosphate isomerase(Tpi)located on the Z chromosome,were used to analyze the subtypes of Spodoptera frugiperda in Sichuan province.【Result】The identification results based on COⅠgene showed that 50samples collected from Sichuan were all′corn strain′.Subsequently,Spodoptera frugiperda fed by artificial diet,maize and rice seedlings until the third generation.It was found that the Spodoptera frugiperda fed on artificial diet and maize seedlings were all′corn strain′,and the samples from rice seedlings were partially differentiated into′rice strain′.But analysis based on the Tpi gene showed that all the samples were′corn strain′.【Conclusion】The results indicated that the Spodoptera frugiperda invading Sichuan province was not a single subtype,and there were some′heterozygous′.The main host plants of Spodoptera frugiperda,such as corn and rice,were widely planted in Sichuan province.Clarifying the genetic characteristics of Spodoptera frugiperda can provide a scientific basis for the agricultural sector to develop targeted prevention and control measures.

关 键 词：草地贪夜蛾 不同寄主 分子标记 亚型 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]