纳米金-适配体电化学传感器用于环境水样中双酚A检测  

Gold nanoparticles-aptamer electrochemical sensor for detection of bisphenol A in environmental waters

作　　者：孟雪洁 张瑜 刘京华 谭峰[1] MENG Xuejie;ZHANG Yu;LIU Jinghua;TAN Feng(Key Laboratory of Industrial Ecology and Environmental Engineering(MOE),School of Environmental Science and Technology,Dalian University of Technology,Dalian,116024,China;Dalian Customs Comprehensive Technical Service Center,Dalian,116001,China)

机构地区：[1]工业生态与环境工程教育部重点实验室,大连理工大学环境学院,大连116024 [2]大连海关综合技术服务中心,大连116001

出　　处：《环境化学》2023年第2期379-387,共9页Environmental Chemistry

基　　金：辽宁省自然科学基金(2020-MS-124);中央高校基本科研业务费(DUT20YG123)资助。

摘　　要：制备了一种用于检测双酚A的纳米金-适配体电化学传感器.采用恒电位沉积的方法在玻碳电极表面制备金纳米颗粒提高电极的比表面积及导电性,通过Au—S键作用将末端带有巯基、可特异性识别双酚A的适配体固定在电极表面,以[Fe(CN)_(6)]^(3-/4-)作为氧化还原探针,通过循环伏安法和交流阻抗法对纳米金-适配体电化学传感器的检测性能进行研究,优化了检测性能的影响因素.当金纳米颗粒沉积时间为400 s、适配体浓度为1μmol·L^(-1)、富集时间为50 min、检测溶液pH 7.0时,电极阻抗响应与双酚A浓度在1×10^(-9)—5×10^(-6)mol·L^(-1)范围内呈良好线性关系.发展的传感器具有良好的重现性、稳定性和抗干扰性,并成功应用于环境水样中双酚A的检测,回收率87.4%—110.0%.A gold nanoparticles-aptamer electrochemical sensor was prepared for the determination of bisphenol A(BPA).Gold nanoparticles were deposited on the surface of glassy carbon electrode by potentiostatic deposition to improve the specific surface area and electrical conductivity of the electrode and the bisphenol A aptamer with a terminal sulfhydryl group that can specifically bind bisphenol A was immobilized onto on the modified electrode surface through the interaction between Au—S bond.Using[Fe(CN)_(6)]^(3-/4-)as a redox probe,the performance of gold nanoparticles-aptamer electrochemical sensor was investigated by cyclic voltammetry and electrochemical impedance spectroscopy,and the factors affecting the sensor performance were optimized.When the deposition time of gold nanoparticles was 400 s,the concentration of aptamer was 1μmol·L^(-1),the enrichment time was 50 min at solution pH of 7.0,there was a linear relationship between the impedance response and bisphenol A concentrations ranging from 1×10^(-9)mol·L^(-1)to 5×10^(-6)mol·L^(-1).The prepared electrode had good reproducibility,stability and anti-interference,and was successfully applied to the detection of bisphenol A in environmental water samples with recoveries of87.4%—110.0%.

关 键 词：适配体 双酚A 电化学检测 金纳米颗粒 

分 类 号：X832[环境科学与工程—环境工程]