细菌-多肽纳米颗粒复合体的超微结构表征  

Ultrastructure of bacteria-peptide nanoparticle complex

作　　者：卿光超 郭宏博[1] 张宇轩 李宪磊 甘雅玲[1] 罗阳 梁兴杰[1] QING Guang-chao;GUO Hong-bo;ZHANG Yu-xuan;LI Xian-lei;GAN Ya-ling;LUO Yang;LIANG Xing-jie(National Center for Nanoscience and Technology,Beijing 100190;School of Medicine of Chongqing University,Chongqing 400044,China)

机构地区：[1]国家纳米科学中心,北京100190 [2]重庆大学医学院,重庆400044

出　　处：《电子显微学报》2023年第1期49-52,共4页Journal of Chinese Electron Microscopy Society

基　　金：国家重点研发计划项目(No.2021YFA1201000);国家自然科学基金重点项目(No.32030060);国家自然科学基金面上资助项目(Nos.82072383,81871733);国家杰出青年科学基金资助项目(No.82125022)。

摘　　要：细菌感染严重危害人类健康和公共卫生安全。基于抗菌肽(AMP)的多肽纳米颗粒在对抗细菌耐药中展现出巨大潜力,在多肽纳米颗粒与细菌相互作用的过程中,其超微结构形态的变化对阐明多肽纳米颗粒的抑菌机制至关重要。本文开发了一种对细菌具有较好亲和力的多肽纳米颗粒(BPN),并利用扫描电子显微镜(SEM)和透射电子显微镜(TEM)观察了金黄色葡萄球菌(S.aureus)和铜绿假单胞菌(P.aeruginosa)在结合BPN前后的超微结构形态。结果显示,与BPN共孵育后,细菌表面覆盖着明显的凸起状物质,表明BPN可以有效结合在S.aureus和P.aeruginosa表面。Bacterial infection is a life-threatening disease and seriously endangers public health. Antimicrobial peptide-based nanomaterials show great potential in combating bacterial antibiotic resistance. The bacterial ultrastructural change after treatment is conducive to elucidate the antibacterial mechanism of peptide-based nanoparticles. Here, we use SEM and TEM to observe the ultrastructure of Staphylococcus aureus(S. aureus) and Pseudomonas aeruginosa(P. aeruginosa) when co-incubated with a bacteria-captured peptide nanoparticle(BPN). The results show that bacteria are covered with obvious bulged material after co-incubation with BPN, indicating the effective binding capacity of BPN to S. aureus and P. aeruginosa.

关 键 词：微观形貌 多肽纳米颗粒 细菌 

分 类 号：Q811[生物学—生物工程] Q934